Global proteome survey of human T leukemic cells for systems biology.

机译：用于系统生物学的人类T白血病细胞的全球蛋白质组学调查。

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The reconstruction of signaling network within a single human cell is important for the comprehensive understanding of diverse cellular processes at the systems level. A common approach to do that is by integration of multidimensional "omics" data. In this dissertation, a global survey of a single human Jurkat T leukemic cell line was performed by integrating extensive proteomic and genome-wide transcriptomic profiling, leading to the largest proteome identification to date. The feasibility of using the spectral count method for detecting protein differential regulation was demonstrated, and successfully applied to determine large-scale protein subcellular localization. Moreover, the quantities of several hundreds of proteins in the whole cell and plasma membrane fraction were estimated using the spectral count method. Finally, these omics data sets were integrated with known human protein-protein interactome, generating a first draft of human Jurkat T leukemic protein signaling network, allowing hypotheses generation and validation studies.

机译：在单个人类细胞内重建信号网络对于全面了解系统级别的各种细胞过程非常重要。一种常用的方法是通过集成多维“组学”数据。本论文通过整合广泛的蛋白质组学和全基因组转录组谱分析，对单个人Jurkat T白血病细胞系进行了一项全球调查，从而导致了迄今为止最大的蛋白质组鉴定。证明了使用光谱计数法检测蛋白质差异调节的可行性，并成功地应用于确定大规模蛋白质亚细胞定位。此外，使用光谱计数法估计了整个细胞和质膜部分中数百种蛋白质的数量。最后，将这些组学数据集与已知的人类蛋白质-蛋白质相互作用组整合在一起，生成人类Jurkat T白血病蛋白质信号网络的初稿，从而可以进行假设的产生和验证研究。

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作者
Wu, Linfeng.;
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University of Connecticut.;

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授予单位 University of Connecticut.;
学科 Biology Molecular.;Biology Cell.
学位 Ph.D.
年度 2007
页码 100 p.
总页数 100
原文格式 PDF
正文语种 eng
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专利

1. Global survey of human T leukemic cells by integrating proteomics and transcriptomics profiling. [J] . Wu L, Hwang SI, Rezaul K, Molecular & cellular proteomics: MCP . 2007,第8期

机译：通过整合蛋白质组学和转录组学分析对人类T白血病细胞进行全球调查。
2. Survey of the Human Pancreatic [beta]-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D^sub 1^ in Enhancing Human [beta]-Cell Replication and Function In Vivo [J] . Nathalie Fiaschi-Taesch Todd A Bigatel Brian Sicari Karen K Takane Fatima Salim Silvia Velazquez-Garcia George Harb Karen Selk Irene Cozar-Castellano Andrew F Stewart Diabetes . 2009,第4期

机译：人类胰腺β-细胞G1 / S蛋白质组的调查揭示了Cdk-6和细胞周期蛋白D ^ sub 1 ^在增强人类β-细胞复制和体内功能方面的潜在治疗作用。
3. Survey of the human pancreatic β-cell G1/S proteome reveals a potential herapeutic role for Cdk-6 and cyclin D_1 in enhancing human β-cell replication and function in vivo [J] . Nathalie Fiaschi-Taesch, Todd A. Bigatel, Brian Sicari, Diabetes . 2009,第4期

机译：人类胰腺β细胞G1 / S蛋白质组的调查显示Cdk-6和细胞周期蛋白D_1在体内增强人类β细胞复制和功能中可能具有治疗作用
4. Comparative Analysis of the Global Proteome of Naive and IL-2 Stimulated Human NK Cells [C] . Di Ma, Weifeng Cao, Arvinder Kapur, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：Naive和IL-2刺激人NK细胞全球蛋白质组的比较分析
5. Characterization of the biology of normal and leukemic human hematopoietic stem cells. [D] . Wang, Jean Chuen Yi. 2005

机译：正常和白血病人造血干细胞的生物学特性。
6. Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing Human β-Cell Replication and Function In Vivo [O] . Nathalie Fiaschi-Taesch, Todd A. Bigatel, Brian Sicari, 2009

机译：人类胰腺β细胞G1 / S蛋白质组的调查显示Cdk-6和细胞周期蛋白D1在增强人类β细胞复制和体内功能方面的潜在治疗作用。
7. Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing Human β-Cell Replication and Function In Vivo [O] . Fiaschi-Taesch, Nathalie, Bigatel, Todd A., Sicari, Brian, 2009

机译：人类胰腺β细胞G1 / S蛋白质组的调查显示Cdk-6和细胞周期蛋白D1在增强人类β细胞复制和体内功能方面的潜在治疗作用。

Global proteome survey of human T leukemic cells for systems biology.

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