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Determining the efficacy of a commercially available post-thaw bovine semen sexing kit.

机译：确定市售解冻后牛精液性别鉴定试剂盒的功效。

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摘要

Swaying the bovine sex ratio is beneficial because dairy heifers are significantly more valuable than bull calves, whereas in the beef industry, bull calves are more desirable than females. Currently, there is no inexpensive, reliable, effective method for commercially separating X- and Y-bearing sperm. The objective of these experiments was to determine the efficacy of a commercially available post-thaw bovine semen sexing kit, HeiferPlus(TM), which claims to sway the sex ratio in favor of female calves.;Three trials included the insemination of hyperstimulated cows with Control or HeiferPlus(TM) treated semen, non-surgical embryo collection on Day 7, and a novel combined PCR/Southern blot assay to determine embryo sex. Overall, 271 embryos were collected and a sex was assigned to 265 of them. Chi square analysis showed that the Control group produced a significantly higher proportion (p0.0005) of female embryos than the HeiferPlus(TM) (HP) group (65.0% and 43.0%, respectively). There was no difference in the proportion of transferable versus degenerate embryos and ANOVA showed no difference in the number of ovulations, embryos, and unfertilized oocytes collected from Control versus HP groups. Of the two bulls used in the hyperstimulation studies, one produced an overall higher proportion of females (p0.05), suggesting a bull effect.;Another trial involved the insemination of cows synchronized via OvSynch RTM with fetal sexing via ultrasonography between Days 55-58. Of the 152 cows inseminated, 51.3% (78/152) were pregnant at Day 35. Of the cows inseminated with HP treated semen, 54.5% became pregnant and 48.0% of the Controls were pregnant. HP treated cows resulted in 54.8% male (23/42) and 45.2% female (19/42) fetuses. Control cows had 52.8% male (19/36) and 47.2% female (17/36) fetuses at 58 days gestation. Chi square analysis showed no significant difference in the sex ratio proportions. Semen from six bulls was used in this trial, and pregnancy rates among bulls were not different. Computer automated semen analysis (CASA) showed that there was no significant difference in motility or progressive forward motility of Control versus HP treated semen in any of the bulls.;Results of these studies indicated that HeiferPlus(TM) semen sexing kit did not sway the sex ratio in favor of females in either hyperstimulated or single-ovulating cows. In the hyperstimulated cows, the Control group actually produced a significantly higher proportion of female embryos than the HP group. No differences in embryo production or embryo grade were observed. Further research is needed to investigate the effects of semen incubation or other semen manipulations on the sex ratio of cattle.
机译：改变牛的性别比例是有益的，因为奶牛比公牛犊更有价值，而在牛肉行业，公牛犊比雌牛更可取。当前，没有廉价，可靠，有效的方法来商业分离含X和Y的精子。这些实验的目的是确定市售的解冻后牛精液性鉴定试剂盒HeiferPlus（TM）的功效，该试剂盒声称会影响雌性犊牛的性别比。对照或HeiferPlus™处理的精液，第7天的非手术胚胎收集以及新颖的组合PCR / Southern印迹测定法，以确定胚胎的性别。总体上，收集了271个胚胎，并为其分配了265个性别。卡方分析显示，对照组的女性胚胎比例明显高于HeiferPlus™（HP）组（p <0.0005）（分别为65.0％和43.0％）。可移植胚胎与简并胚胎的比例没有差异，ANOVA显示从对照组和HP组收集的排卵，胚胎和未受精卵母细胞的数量也没有差异。在过度刺激研究中使用的两头公牛中，一头产生了更高比例的雌性（p <0.05），表明有公牛效应。 58。在第35天，受精的152头母牛中，有51.3％（78/152）怀孕。在经过HP治疗的精液中受精的母牛中，有54.5％怀孕，而对照组的48.0％怀孕。经HP处理的母牛产生了54.8％的男性（23/42）和45.2％的女性（19/42）胎儿。对照母牛在妊娠58天时有52.8％的男性（19/36）和47.2％的女性（17/36）胎儿。卡方分析显示性别比例没有显着差异。在该试验中使用了六头公牛的精液，公牛之间的怀孕率没有差异。计算机自动精液分析（CASA）显示，在任何公牛中，对照和精子处理精液的运动性或进行性向前运动没有显着差异。这些研究结果表明，HeiferPlusTM精液性爱试剂盒不会影响过度刺激或单排卵母牛的性别比例偏向雌性。在过度刺激的母牛中，对照组实际上产生的雌性胚胎的比例明显高于HP组。没有观察到胚胎产量或胚胎等级的差异。需要进行进一步的研究以研究精液孵育或其他精液操作对牛性别比的影响。

著录项

  • 作者

    Curry, Erin.;

  • 作者单位

    Clemson University.;

  • 授予单位 Clemson University.;
  • 学科 Biology Animal Physiology.;Agriculture Animal Pathology.
  • 学位 M.S.
  • 年度 2007
  • 页码 90 p.
  • 总页数 90
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生理学;动物医学（兽医学）;
  • 关键词

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