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首页> 外文学位 >Polyhydroxybutyrate (PHB) production in transgenic Arabidopsis thaliana seeds.
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Polyhydroxybutyrate (PHB) production in transgenic Arabidopsis thaliana seeds.

机译：转基因拟南芥种子中的聚羟基丁酸酯（PHB）生产。

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摘要

Polyhydroxybutyrate (PHB) belongs to a class of biodegradable polymers, which has properties similar to conventional plastics. Ralstronia eutropha naturally produces PHB using acetyl-CoA. Acetyl-CoA is also abundantly found in developing seeds of higher plants, where oil synthesis takes place. Three genes responsible for PHB synthesis were isolated from R. eutropha. Two gene constructs were prepared, driven by seed-specific promoters, to drive PHB synthesis in cytoplasm and leucoplast of developing seeds. The cytosolic construct was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation. Homozygous lines with single copy transgenes were developed and characterized at molecular level. Transgenic seeds were analyzed by Gas Chromatography and found to accumulate 0.772 +/- 0.006% PHB (dry weight). Furthermore, phb genes were over-expressed in E. coli and recombinant Phb proteins were purified. Successfully purified Phb proteins were used for immunization into rabbit hosts to produce polyclonal antibodies.
机译：聚羟基丁酸酯（PHB）属于一类可生物降解的聚合物，其性质与常规塑料相似。 Ralstronia eutropha使用乙酰辅酶A自然产生PHB。乙酰辅酶A还广泛存在于高等植物的发育种子中，在那里进行油的合成。从富营养罗非鱼中分离出负责PHB合成的三个基因。制备了两个由种子特异性启动子驱动的基因构建体，以驱动发育中的种子的细胞质和白质中的PHB合成。通过农杆菌介导的转化将胞质构建体引入拟南芥。具有单拷贝转基因的纯合品系被开发并在分子水平上表征。通过气相色谱法分析转基因种子，发现其积累了0.772 +/- 0.006％的PHB（干重）。此外，phb基因在大肠杆菌中过表达，并纯化了重组Phb蛋白。成功纯化的Phb蛋白用于免疫兔宿主以产生多克隆抗体。

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  • 作者

    Suriyamongkol, Pornpa.;

  • 作者单位

    University of Alberta (Canada).;

  • 授予单位 University of Alberta (Canada).;
  • 学科 Agriculture Plant Pathology.
  • 学位 M.Sc.
  • 年度 2006
  • 页码 191 p.
  • 总页数 191
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 老年病学;
  • 关键词

  • 入库时间 2022-08-17 11:40:03

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