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首页> 外文会议>UKM FST Postgraduate Colloquium >Isolation of Persicaria minor Sesquiterpene Synthase Promoter and its Deletions for Transgenic Arabidopsis thaliana
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Isolation of Persicaria minor Sesquiterpene Synthase Promoter and its Deletions for Transgenic Arabidopsis thaliana

机译：患有Persicaria次要倍二萜合酶启动子的分离及其对转基因拟南芥的缺失

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摘要

Sesquiterpene synthase (SS) catalyzes the formation of sesquiterpenes from farnesyl diphosphate (FDP) via carbocation internediates. In this study, the promoter region of sesquiterpene synthase was isolated from Persicaria minor to identify possible cis- acting elements in the promoter. The full-length PmSS promoter of P. minor is 1824-bp sequences. The sequence was analyzed and several putative cis-acting regulatory elements were identified. Three cis-acting regulatory elements were selected for deletion analysis which are cis-acting element involved in wound responsiveness (WUN), cis acting element involved in defense and stress responsiveness (TC) and cis-acting element involved in ABA responsiveness (ABRE). Series of deletions were conducted to assess the promoter activity producing three truncated fragments promoter; Prom 2 1606 -bp, Prom 3 1144- bp, and Prom 4 921 -bp. The full-length promoter and its deletion series were cloned into the pBGWFS7 vector which contain β-glucuronidase (GUS) gene and green fluorescent protein (GFP) as the reporter gene. All constructs were successfully transformed into Arabidopsis thaliana based on PCR of positive BASTA resistance plants.
机译：SesquiterPene合酶（SS）催化通过携带碳统计数据从法呢基二磷酸（FDP）的形成倍半萜烯。在该研究中，从Persicaria次要分离倍二萜合酶的启动子区域以识别启动子中可能的顺应元素。 P.Minor的全长PMS启动子是1824年-BP序列。分析序列，鉴定了几种推定的顺式作用调节元件。三个顺式作用调节元件被选择用于缺失分析，其是顺式作用参与伤口响应性（WUN）元件，顺作用参与防御和应力响应（TC）和顺式作用参与ABA响应性（ABRE）元素的元素。进行系列缺失，以评估产生三个截碎片启动子的启动子活性; PROM 2 1606 -BP，PROM 3 1144-BP，以及PROM 4 921-BP。全长启动子及其删除系列被克隆到PBGWFS7载体中，含有β-葡萄糖醛酸酶（GUS）基因和绿色荧光蛋白（GFP）作为报告基因。所有构建体都以阳性巴斯塔抗性植物的PCR成功转化为拟南芥。

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