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首页> 外文学位 >Creating an embryonic kidney: The role of bone morphogenetic protein signaling during patterning and differentiation of pronephric tissue in Xenopus laevis.
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Creating an embryonic kidney: The role of bone morphogenetic protein signaling during patterning and differentiation of pronephric tissue in Xenopus laevis.

机译：创建胚胎肾脏：非洲爪蟾前肾组织的模式和分化过程中骨形态发生蛋白信号传导的作用。

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摘要

Bone Morphogenetic Proteins (BMPs) mediate diverse developmental processes including embryogenesis, organ morphogenesis as well as regeneration. Although studies in transgenic mice have implicated the necessity of BMPs during later stages of mammalian nephrogenesis, a role during patterning and differentiation of the pronephros, has yet to be identified. In order to address this gap, we examined the effect of BMP mis-regulation on differentiation of the pronephric kidney in Xenopus laevis. Because BMP signaling is required during early developmental events such as axis specification and mesoderm patterning, it is imperative to maintain temporal and spatial control of BMP manipulations. To achieve this, we utilized hormone-inducible constructs designed to either up or down-regulate BMP signaling. Our results show that down-regulation of the BMP pathway leads to undifferentiated and non-functional tubules and duct, but has no effect on glomal development. Interestingly, we noted an increase in expression of the unknown tubule antigen 3G8 in the absence of BMP signaling. Up-regulation of the BMP pathway results in a loss of all nephric markers examined including glomal transcripts. We demonstrate that the effect of BMP down-regulation on the pronephric kidney is not due to a primary effect on general mesoderm development; however this may be the case for BMP up-regulation. Our results further elucidate the molecules involved in creating and patterning the pronephros, a system whose accurate formation is critical for proper development of subsequent nephric structures.
机译：骨形态发生蛋白（BMP）介导各种发育过程，包括胚胎发生，器官形态发生以及再生。尽管对转基因小鼠的研究表明在哺乳动物肾生成的后期需要BMPs，但尚未确定其在前肾的模式和分化中的作用。为了解决这一差距，我们检查了非洲爪蟾中BMP调节异常对肾前肾分化的影响。由于在早期发育事件（例如轴规格和中胚层模式）期间需要BMP信号传导，因此必须保持BMP操作的时间和空间控制。为了实现这一目标，我们利用了激素诱导型构建物，旨在上调或下调BMP信号传导。我们的结果表明，BMP途径的下调导致未分化和无功能的肾小管和导管，但对阴郁发育没有影响。有趣的是，我们注意到在缺乏BMP信号的情况下，未知肾小管抗原3G8的表达增加。 BMP途径的上调导致所有检查的肾标志物（包括转录物）丢失。我们证明BMP下调对前肾的影响不是由于对中胚层发育的主要影响。但是BMP上调可能就是这种情况。我们的结果进一步阐明了创建和构图前肾的分子，该系统的准确形成对于后续肾结构的正常发育至关重要。

著录项

  • 作者

    Bracken, Christina M. E.;

  • 作者单位

    Tufts University.;

  • 授予单位 Tufts University.;
  • 学科 Biology Molecular.;Biology Animal Physiology.;Biology Cell.
  • 学位 M.S.
  • 年度 2007
  • 页码 279 p.
  • 总页数 279
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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