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Xenopus laevis bone morphogenetic proteins and use thereof

机译:非洲爪蟾的骨形态发生蛋白及其用途

摘要

Disclosed are (1) a Xenopus laevis bone morphogenetic protein (BMP), (2) a DNA comprising a DNA segment coding for a Xenopus laevis BMP, (3) a transformant bearing a DNA comprising a DNA segment coding for a Xenopus laevis BMP and (4) a method for preparing the Xenopus laevis BMP which comprises culturing the described in (3), producing and accumulating the protein in a culture, and collecting the protein thus obtained. Cells transfected or transformed with the DNA allow large amounts of the Xenopus laevis BMP mature peptides to be produced, which causes the advantageous production of the peptides, which promote the synthesis of proteoglycan and can also be utilized for analysis of the mechanism of organism, particularly human bone-cartilage morphogenetic reaction, and as therapeutic agents for osteoporosis.
机译:公开了(1)非洲爪蟾骨形态发生蛋白(BMP),(2)包含编码非洲爪蟾BMP的DNA片段的DNA,(3)带有DNA的转化体,所述DNA包含编码非洲爪蟾BMP的DNA片段,和(4)制备非洲爪蟾BMP的方法,该方法包括培养(3)中所述的方法,在培养物中产生和积累蛋白质,并收集由此获得的蛋白质。用DNA转染或转化的细胞可产生大量非洲爪蟾BMP成熟肽,这可有利地产生该肽,从而促进蛋白聚糖的合成,还可用于分析生物体的机制,特别是人骨软骨形态发生反应,并作为骨质疏松症的治疗剂。

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