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Novel strategies for the detection of pathogens in drinking water.

机译：检测饮用水中病原体的新策略。

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摘要

To protect public health, detection methods have been developed to monitor drinking water for pathogens. The goal of this dissertation is to evaluate and utilize novel methods that enhances detection and further reduces the risk of waterborne pathogens.;The study in Appendix A developed a method to monitor the microbial quality of treated drinking water at the tap utilizing point-of-use (POU) filter. Tap water supplies were monitored in vending machines throughout Southern Arizona using solid block carbon (SBC) filters as a monitoring tool. Out of 48 SBC filters 54.2% were positive for at least one organism. The number of filters positive for total coliforms, E. coli, Enterococci, and enterovirus was 13, 5, 19, and 3, respectively, corresponding to 27.1%, 10.4%, 39.6%, and 6.3% of the total filters. These results suggest that the SBC filter can be used to monitor large volumes of treated drinking water and detect the incidence of indicators and pathogens.;The study in Appendix B evaluated the fate of infectious prions in multiple water sources quantitatively utilizing a method that only detects infectious prions. A reduction of PrPSc was observed at 25°C and 37°C ranging between 0.41-log10 and 1.4-log10 after 1 week. After 8 weeks at 25°C and 37°C, inactivation ranged between 1.65-log 10 and 2.15-log10. A maximum rate of inactivation in water occurred at 50°C, ranging from 2.0-log10 and 2.51-log 10 after one week. The results from all types of water suggest that dissolved organic matter and temperature influence PrPSc infectivity.;The study in Appendix C evaluated real-time sensors for monitoring microbial contaminants. Most sensor parameters evaluated exhibited an increase in sensor response to an increase in E. coli concentrations. Responses to E. coli concentrations at or below 103 cfu/mL were very low due to near background levels, and responses to concentrations above 106 cfu/mL exceeded threshold levels for sensors that use light scattering methods due to saturation in the flow cell. The data produced effectively shows that the sensors evaluated could be used to monitor microbial intrusion events in water distribution systems.
机译：为了保护公众健康，已经开发出检测方法来监测饮用水中的病原体。本文的目的是评估和利用能够提高检测效率并进一步降低水源性病原体风险的新颖方法。附录A中的研究提出了一种方法，该方法利用水位监测点监测自来水的微生物质量使用（POU）过滤器。使用固态碳（SBC）过滤器作为监测工具，在整个亚利桑那南部的自动售货机中对自来水进行了监测。在48个SBC过滤器中，至少一种生物体中54.2％呈阳性。总大肠菌群，大肠杆菌，肠球菌和肠病毒阳性的过滤器数量分别为13、5、19和3，分别占总过滤器的27.1％，10.4％，39.6％和6.3％。这些结果表明，SBC过滤器可用于监测大量处理后的饮用水并检测指示剂和病原体的发生率;附录B中的研究使用仅能检测出污染物的方法定量评估了多种水源中感染性ions病毒的去向。传染性病毒。 1周后，在25°C和37°C下观察到PrPSc的降低，介于0.41-log10和1.4-log10之间。在25°C和37°C下放置8周后，失活的范围为1.65-log 10和2.15-log10。水中的最大失活速率发生在50°C，一周后范围为2.0-log10和2.51-log 10。所有类型的水的结果表明，溶解的有机物和温度会影响PrPSc的感染性。附录C中的研究评估了用于监测微生物污染物的实时传感器。评估的大多数传感器参数均表现出对大肠杆菌浓度增加的传感器响应增加。由于接近本底水平，对103 cfu / mL或以下的大肠杆菌浓度的响应非常低，而对于使用光散射方法的传感器，由于流通池中的饱和度，对106 cfu / mL以上的浓度的响应超过了阈值水平。有效产生的数据表明，所评估的传感器可用于监控配水系统中的微生物入侵事件。

著录项

  • 作者

    Miles, Syreeta Lynn.;

  • 作者单位

    The University of Arizona.;

  • 授予单位 The University of Arizona.;
  • 学科 Biology Microbiology.;Environmental Health.;Environmental Sciences.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 137 p.
  • 总页数 137
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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