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Autofluorescence spectroscopy of epithelial tissue.

机译：上皮组织的自发荧光光谱。

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摘要

Fluorescence diagnosis of epithelial precancer is based on the difference in spectral characteristics between normal and precancerous tissue. However, the bulk autofluorescence measured by conventional methods do not provide accurate diagnostic information such as structure and metabolism of tissue because the signal is a mixture of the fluorescence from different tissue layers. In this study, we demonstrated that depth-resolved fluorescence measurement can isolate the fluorescence signals from different tissue layers and provide more accurate information for tissue diagnosis.; We instrumented a confocal fluorescence spectroscopy system with single-photon excitation from 355--473 nm to investigate the layered structure and biochemistry of various epithelial tissues. It was found that strong keratin fluorescence from the keratinized epithelial layer creates severe interference in the assessment of NADH, FAD and collagen fluorescence. The depth-resolved fluorescence spectra excited at 355 nm produced sufficient contrast to resolve such fine structure, and the depth-resolved redox ratio from non-keratinized epithelium (the ratio of NADH fluorescence excited at 355nm over FAD fluorescence excited at 457nm) showed high correlation with tissue pathology. Furthermore, we demonstrated that confocal time-resolved measurement with single excitation from uv to violet can potentially provide accurate information for tissue diagnosis because different tissue layers exhibited different fluorescence time decays and the time decays of epithelial fluorescence were sensitive indicators of cellular metabolism.; The depth-resolved fluorescence measurement was also achieved using two-photon excitation from 710--810 nm. The two-photon excited fluorescence (TPEF) signals from the keratinized epithelial layer, non-keratinized epithelium and underlying stroma exhibited different spectral characteristics providing information on biomorphology and biochemistry of epithelial tissue. The second harmonic generation (SHG) signals served as a sensitive indicator of collagen to separate the epithelial layer from underlying stroma. The results also demonstrated the potential of depth-resolved TPEF and SHG in determining the pathology of epithelial tissue.
机译：上皮前癌的荧光诊断是基于正常组织和癌前组织之间光谱特征的差异。然而，由于信号是来自不同组织层的荧光的混合物，因此通过常规方法测量的整体自发荧光不能提供准确的诊断信息，例如组织的结构和新陈代谢。在这项研究中，我们证明了深度分辨荧光测量可以隔离来自不同组织层的荧光信号，并为组织诊断提供更准确的信息。我们在355--473 nm范围内用单光子激发仪检测了共聚焦荧光光谱系统，以研究各种上皮组织的分层结构和生化特性。发现来自角化上皮层的强角蛋白荧光在NADH，FAD和胶原蛋白荧光的评估中产生严重干扰。在355 nm激发的深度分辨荧光光谱产生了足以分辨这种精细结构的对比度，非角质化上皮的深度分辨氧化还原比（355nm激发的NADH荧光与457nm激发的FAD荧光的比例）显示出高度相关性与组织病理学有关。此外，我们证明了从紫外光到紫光单次激发的共聚焦时间分辨测量可以潜在地为组织诊断提供准确的信息，因为不同的组织层表现出不同的荧光时间衰减，而上皮荧光的时间衰减是细胞代谢的敏感指标。还使用710--810 nm的双光子激发实现了深度分辨荧光测量。来自角化上皮层，未角化上皮和下层间质的双光子激发荧光（TPEF）信号表现出不同的光谱特征，从而提供了上皮组织的生物形态和生物化学信息。二次谐波（SHG）信号用作胶原蛋白的敏感指示剂，可将上皮层与下面的基质分开。结果还证明了深度分辨的TPEF和SHG在确定上皮组织病理学方面的潜力。

著录项

  • 作者

    Wu, Yicong.;

  • 作者单位

    Hong Kong University of Science and Technology (People's Republic of China).;

  • 授予单位 Hong Kong University of Science and Technology (People's Republic of China).;
  • 学科 Engineering Biomedical.; Engineering Electronics and Electrical.
  • 学位 Ph.D.
  • 年度 2007
  • 页码 151 p.
  • 总页数 151
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物医学工程;无线电电子学、电信技术;
  • 关键词

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