• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Molecular approaches for characterization of biodegradation genes expressed during microbial colonization on decay-resistant and non-resistant woods in forest soil.
【24h】

Molecular approaches for characterization of biodegradation genes expressed during microbial colonization on decay-resistant and non-resistant woods in forest soil.

机译：表征微生物降解过程中在森林土壤中抗​​腐和非抗腐木材上微生物定殖表达的分子方法。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

White and brown-rot fungi damage wood by production of enzymes that attack the structural components. The objective of this study was to characterize decay related genes and proteins that are expressed on three different wood types undergoing decay over 18 months. Variation in gene expression, presence of decay enzymes and proteins were determined for untreated pine (non-resistant), western red cedar (naturally durable), and alkaline copper quaternary (ACQ) treated pine (chemically resistant) exposed in a soil decay bed test. Decay was assessed by visual decay ratings, dynamic modulus of elasticity (MOE), and microscopy. There were no significant differences in decay between cedar and ACQ-treated pine over the 18 month period. However, there were significant differences in decay between pine and cedar and between pine and ACQ-treated pine. The fungal mycelia penetrated the cell walls of pine and were continually observed over 18 months, but were not observed in cedar or ACQ-treated pine. Basidiomycetes containing decay genes lignin peroxidase (Lip), manganese peroxidase (Mnp), and laccase (Lcc) were detected on pine and ACQ-treated pine which also a greater diversity of fungi had compared to cedar. Phlebia radiata specific-lignin peroxidase and manganese peroxidase genes were expressed approximately equally on pine and ACQ-treated pine at most sampling times. The expression of P. radiata specific Lcc was higher on ACQ-treated pine than untreated pine. No basidiomycete genes were expressed and only a few basidiomycetes were identified on cedar, which also showed little decay. ACQ-treated pine also showed little decay, however basidiomycetes were present and active. Proteins were first detected on pine and ACQ-treated pine at 6 months and continued to increase through 18 months, but were not detected on cedar until 14 months exposure. There were greater numbers of total proteins on pine than on cedar and ACQ-treated pine at each time period. Decay genes were only found on pine but not on cedar and ACQ-treated pine. Additionally, the types of proteins and their score were different among the three wood types. From these results, the natural durability of cedar reduced the wood decay community and its activities. It appears that ACQ-treated wood did not stop the production of the decay enzymes but the chemical treatment did inhibit the effectiveness of the wood decay genes.
机译：白腐病和褐腐病真菌通过产生攻击结构成分的酶来破坏木材。这项研究的目的是鉴定与衰变相关的基因和蛋白质，这些基因和蛋白质在经历18个月以上腐烂的三种不同木材上表达。确定了在土壤腐烂试验中暴露的未经处理的松树（非抗性），西部红柏（自然耐久）和经碱性铜季铵盐（ACQ）处理的松树（化学抗性）的基因表达变化，衰变酶和蛋白质的存在。通过视觉衰减等级，动态弹性模量（MOE）和显微镜来评估衰变。在18个月的时间里，雪松和ACQ处理的松树之间的腐烂没有显着差异。但是，在松树和雪松之间以及在松树和ACQ处理过的松树之间，衰变存在显着差异。真菌菌丝体穿透了松树的细胞壁，并在18个月内不断观察到，但在雪松或ACQ处理的松树中未观察到。在松树和经ACQ处理的松树上检测到含有衰变基因木质素过氧化物酶（Lip），锰过氧化物酶（Mnp）和漆酶（Lcc）的担子菌，与雪松相比，真菌的多样性也更大。在大多数采样时间，松树和ACQ处理过的松树上平均辐射白粉病菌木质素过氧化物酶和锰过氧化物酶基因均相等。在ACQ处理的松树上，辐射松特异性Lcc的表达高于未处理的松树。没有表达担子菌基因，并且在雪松上仅鉴定了少数担子菌，其衰减也很小。经ACQ处理的松树也未见腐烂，但存在担子菌且活跃。最初在6个月的松树和经ACQ处理的松树上检测到蛋白质，并在18个月内持续增加，但直到14个月才在雪松上检测到蛋白质。在每个时间段，松树上的总蛋白质数量均大于雪松和ACQ处理过的松树上的蛋白质总量。衰变基因仅在松树上发现，而在雪松和ACQ处理的松树上则没有。此外，三种木材类型中蛋白质的类型及其得分不同。从这些结果来看，雪松的自然耐久性降低了木材腐烂群落及其活动。似乎用ACQ处理的木材并没有阻止衰变酶的产生，但是化学处理确实抑制了木材衰变基因的有效性。

著录项

  • 作者

    Kang, Youngmin.;

  • 作者单位

    Mississippi State University.;

  • 授予单位 Mississippi State University.;
  • 学科 Biology Molecular.;Environmental Sciences.;Biology Microbiology.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 197 p.
  • 总页数 197
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号