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首页> 外文期刊>Forest Pathology >Comparison of the gene expression profiles of resistant and non-resistant Japanese black pine inoculated with pine wood nematode using a modified LongSAGE technique.
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Comparison of the gene expression profiles of resistant and non-resistant Japanese black pine inoculated with pine wood nematode using a modified LongSAGE technique.

机译：使用改良的LongSAGE技术比较接种松木线虫的抗性和非抗性黑松的基因表达谱。

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摘要

The Japanese black pine (Pinus thunbergii) is highly susceptible to pine wilt disease caused by the pine wood nematode (PWN; Bursaphelenchus xylophilus). To cope with this disease, researchers and tree breeders selected PWN-resistant individuals in a previous breeding program. In an attempt to understand the mechanisms of resistance in the Japanese black pine, we created four LongSAGE (serial analysis of gene expression) libraries. A total of 20818 tags were studied, including 5194 tags from a PWN inoculated resistant pine, 5218 a non-inoculated resistant pine, 5194 an inoculated non-resistant pine, and 5212 a non-inoculated non-resistant pine. The analysis of the libraries indicated that 14 tag species were significantly up-regulated (e.g., pathogenesis-related proteins 2 and 4, osmotin, lipoxygenase, and chalcone synthase), and nine were down-regulated (eukaryotic translation initiation factor SUI1, translationally controlled tumor protein, and xyloglucan endotransglycosylase) by the PWN inoculation in both the resistant and non-resistant pines. On the other hand, 38 tag species were significantly expressed at a higher level only in the resistant pine (catalase, dienelactone hydrolase family protein) and 25 were expressed at a higher level in the non-resistant pine (pathogenesis-related proteins 1, 2, and 3, and leucoanthocyanidin dioxygenase). These differentially expressed genes are presumed to reflect some of the differences between the resistant and non-resistant pines. Our results provide valuable information on the complex responses induced in the resistant and non-resistant pine trees in response to PWN invasion.
机译：日本黑松（ Punus thunbergii ）对由松木线虫（PWN; Bursaphelenchus xylophilus ）引起的松枯病高度敏感。为了应对这种疾病，研究人员和树木育种者在先前的育种计划中选择了耐PWN的个体。为了了解日本黑松的抗性机制，我们创建了四个LongSAGE（基因表达的序列分析）库。共研究了20818个标签，包括来自PWN接种的抗性松树的5194个标签，5218个未接种的抗性松树，5194个接种的非抗性松树和5212个未接种的非抗性松树。对文库的分析表明，有14种标签物种显着上调（例如，与病程相关的蛋白2和4，渗透蛋白，脂氧合酶和查尔酮合酶），而有9种则被下调（真核翻译起始因子SUI1，受翻译控制）抗性和非抗性松树中通过PWN接种获得肿瘤蛋白和木葡聚糖内切糖苷酶）。另一方面，仅在抗性松树中有38种标签物种显着高水平表达（过氧化氢酶，二内酯水解酶家族蛋白），在非抗性松树中有25种标签物种高水平表达（致病相关蛋白1、2 ，3和白花青素双加氧酶）。推测这些差异表达的基因反映了抗性和非抗性松树之间的某些差异。我们的结果提供了有关抗性和非抗性松树对PWN入侵诱导的复杂响应的有价值的信息。

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