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Differential measures of cellular IFN-gamma responses in swine following influenza vaccination

机译：流感疫苗接种后猪中细胞IFN-γ应答的差异测量

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摘要

Live-attenuated influenza virus (LAIV) vaccines provide broader cross-protection than whole-inactivated virus (WIV) vaccines against influenza A virus (IAV) in swine. However, standard correlates of protection, such as serum hemagglutination inhibition (HI) antibody titers, do not accurately predict cross-protective efficacy provided by LAIV vaccines. While the contribution of T cells to IAV immunity is appreciated, data comparing methods to evaluate IFN-g production by IAV-specific T cells is limited. To understand the differential immunogenicity between LAIV and WIV vaccines as it relates to induction of T cell responses, IFN-g production by peripheral T cells following antigen restimulation was assessed post-vaccination. ELISpot assays used to enumerate IAV-specific IFN-g secreting cells (SC) in peripheral blood 42 days post-vaccination (dpv) indicated that WIV-vaccinated pigs had a greater number of IFN-g SC compared to LAIV vaccinated pigs, regardless of recall IAV used. In LAIV vaccinates there was no change in the number of IFN-g SC to homologous IAV when comparing responses at 77 dpv to 42 dpv, but a decrease over time in WIV vaccinates. Pig age at time of WIV vaccination significantly effected peripheral IAV-specific IFN-g recall responses. In only one instance were statistical differences between vaccine groups different between the ELISpot and ELISA, in all other cases, the same conclusion was made in regards to responses. Collectively, these data indicate that peripheral IAV-specific IFN-g recall responses are not predictive of LAIV vaccination status, nor a useful surrogate for predicting cross-protection. However, the evaluation of IFN-g recall responses may be useful for investigating factors, such as animal age or vaccine formulation that can affect immune responses to WIV vaccination.
机译：减毒活流感病毒（LAIV）疫苗比针对猪中的甲型流感病毒（IAV）的全灭活病毒（WIV）疫苗具有更广泛的交叉保护。但是，保护的标准关联（例如血清血凝抑制（HI）抗体效价）不能准确预测LAIV疫苗提供的交叉保护功效。尽管赞赏T细胞对IAV免疫的贡献，但是用于评估IAV特异性T细胞产生IFN-g的方法的数据比较方法是有限的。为了理解LAIV和WIV疫苗之间的差异性，因为它与T细胞应答的诱导有关，在疫苗接种后评估了抗原再刺激后外周T细胞产生的IFN-g。疫苗接种（dpv）后42天用来计数外周血中IAV特异性IFN-g分泌细胞（SC）的ELISpot分析表明，与LAIV疫苗接种的猪相比，WIV疫苗接种的猪的IFN-g SC数量更多回忆起使用过的IAV。在LAIV疫苗中，比较77 dpv与42 dpv的反应时，相对于同源IAV的IFN-g SC数量没有变化，但WIV疫苗随时间的减少。 WIV疫苗接种时的猪龄显着影响外周IAV特异性IFN-g召回反应。在只有一种情况下，疫苗组之间的统计学差异在ELISpot和ELISA之间有所不同，在所有其他情况下，关于反应的结论是相同的。总体而言，这些数据表明，外周IAV特异性IFN-g召回反应不能预测LAIV疫苗的接种状况，也不能用于预测交叉保护。但是，对IFN-g召回反应的评估可能对调查可能影响WIV疫苗免疫反应的因素（例如动物年龄或疫苗制剂）有用。

著录项

  • 作者

    Olson, Zahra F.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Immunology.;Virology.
  • 学位 M.S.
  • 年度 2014
  • 页码 48 p.
  • 总页数 48
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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