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首页> 外文学位 >Citric acid production by Aspergillus niger strains grown on corn substrates from ethanol fermentation.
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Citric acid production by Aspergillus niger strains grown on corn substrates from ethanol fermentation.

机译：黑曲霉菌株在乙醇发酵下在玉米底物上生长的柠檬酸生产。

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摘要

Citric acid is an important specialty chemical which can be synthesized biologically. It has a number of commercial applications including its use in foods, pharmaceuticals and other industries. In this study, the coproducts resulting from ethanol fermentation of corn were tested for their suitability to be utilized as substrates for citric acid production using solid-state fermentation or surface fermentation. These coproducts include dried corn distillers grains with solubles, wet corn distillers grains, thin stillage and condensed corn distillers solubles. Seven citric acid-producing strains of the fungus Aspergillus niger were selected and screened for their ability to produce citric acid from these corn-based substrates. The treatments of the substrates include autoclaving and mild-acid hydrolysis. In addition, the effects of 3% (v/v) methanol addition and 30 mM KH2 PO4 supplementation were also studied. The concentration of citric acid was analyzed by a coupled enzyme assay. It was found that A. niger ATCC 9142 produced the highest level of citric acid on solid substrates including dried distillers grains with solubles and wet distillers grains. On the other hand, A. niger ATCC 12846 and ATCC 26550 produced the highest biomass level on dried distillers grains with solubles and wet distillers grains, respectively. The effects of methanol and phosphate supplementation on citric acid and biomass production were strain-dependent. It was also found that A. niger ATCC 201122 was the most effective strain for citric acid production on liquid substrates including thin stillage and condensed distillers solubles. A. niger ATCC 201122 also produced the highest specific productivity and citric acid yield on the liquid substrates. Moreover, A. niger ATCC 9029 and ATCC 10577 produced the highest biomass level on thin stillage and condensed distillers solubles, respectively. It was concluded that A. niger strains could use corn-based coproducts from ethanol fermentation as substrates for citric acid production.
机译：柠檬酸是可以生物合成的重要的特种化学品。它具有许多商业应用，包括在食品，药品和其他行业中的使用。在这项研究中，测试了玉米乙醇发酵制得的副产物是否适合用作固态发酵或表面发酵生产柠檬酸的底物。这些副产品包括具有可溶物的干玉米酒糟，湿玉米酒糟，稀釜馏物和浓缩玉米酒糟。选择了七个产生柠檬酸的黑曲霉菌菌株，并筛选了它们从这些基于玉米的底物中产生柠檬酸的能力。底物的处理包括高压灭菌和弱酸水解。此外，还研究了添加3％（v / v）甲醇和补充30 mM KH2 PO4的效果。通过偶联酶测定法分析柠檬酸的浓度。发现黑曲霉ATCC 9142在固体基质上产生最高水平的柠檬酸，所述固体基质包括具有可溶物的干酒糟和湿酒糟。另一方面，黑曲霉ATCC 12846和ATCC 26550分别在具有可溶物的干酒糟和湿酒糟中产生了最高的生物量水平。补充甲醇和磷酸盐对柠檬酸和生物量生产的影响取决于菌株。还发现黑曲霉ATCC 201122是在液体底物上生产柠檬酸的最有效菌株，所述液体底物包括稀釜馏物和冷凝的蒸馏器可溶物。黑曲霉ATCC 201122在液体基质上也产生了最高的比生产率和柠檬酸产量。此外，黑曲霉ATCC 9029和ATCC 10577分别在稀釜馏物和冷凝蒸馏酒可溶物上产生了最高的生物量水平。结论是，黑曲霉菌株可以使用乙醇发酵的玉米基副产物作为柠檬酸生产的底物。

著录项

  • 作者

    Xie, Gang.;

  • 作者单位

    South Dakota State University.;

  • 授予单位 South Dakota State University.;
  • 学科 Chemistry Biochemistry.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 147 p.
  • 总页数 147
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

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