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首页> 外文学位 >Coenzyme Q10 Biosynthesis in Plants: Is the Polyprenyltransferase an Appropriate Gene Target for the Increased Production of CoQ?
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Coenzyme Q10 Biosynthesis in Plants: Is the Polyprenyltransferase an Appropriate Gene Target for the Increased Production of CoQ?

机译：植物中辅酶Q10的生物合成：多异戊二烯基转移酶是增加CoQ产量的适当基因靶标吗？

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摘要

Coenzyme Q10, CoQ10, is a valuable compound found in all organisms that perform oxidative phosphorylation. Its function and biosynthesis have been well studied in model systems such as E. coli and yeast; however, these are less well understood in plants where CoQ10 participates in a number of other cellular functions such as 'alternative' electron transport associated with the plant response to oxidative stress. CoQ10 biosynthesis in plants shares many similarities with other systems based on gene homology and accumulated biosynthetic intermediates. Understanding CoQ10 biosynthesis and its metabolic engineering in plants is increasingly important due to its use in treating heart and other diseases and its use as an antioxidant in both cosmetics and nutraceuticals. This work reviews the various known and postulated biological functions, the biosynthesis, and the metabolic engineering of CoQ 10 in plants. A reliable HPLC-UV method for the routine quantification of CoQ10 in tobacco leaves which allowed for the description of the occurrence of CoQ10 within a leaf, at different stalk positions of the tobacco plant, and through time in a greenhouse is described. Further, haploid genotypes of burley and flue-cured tobaccos, TN90LC and NC55, respectively, were transformed via Agrobacterium-mediated transformation with the gene encoding the Arabidopsis thaliana 4-hydroxybenzoate polyprenyl diphosphate transferase. Individual T0 transgenic plants were identified as having increased CoQ10 content. A transgenic line from self-fertilized, doubled haploid progeny was identified from each NC55 and TN90LC with increased CoQ10 content, 60% and a 40%, respectively, due to a statistically significant genotype effect. Transgenic lines with improved CoQ10 showed an apparent ability to recover from increasing NaCl stress better than non-transgenics. The transgenics recovered equally as well as non-transgenic tissue culture-derived tobaccos also identified as having increased CoQ10. The transgenic DH1 NC55 line with increased CoQ10 displayed no difference in an isoprenoid profile when monitoring chloroplast-derived isoprenoids by LC-APCI-MS/MS.
机译：辅酶Q10，即CoQ10，是在所有执行氧化磷酸化作用的生物中发现的有价值的化合物。它的功能和生物合成已经在模型系统如大肠杆菌和酵母中得到了很好的研究。然而，在辅酶Q10参与许多其他细胞功能（例如与植物对氧化胁迫的反应相关的“替代”电子传输）的植物中，对这些的了解还很少。辅酶Q10在植物中的生物合成与其他基于基因同源性和积累的生物合成中间体的系统具有许多相似之处。由于CoQ10可用于治疗心脏病和其他疾病，并且在化妆品和营养保健品中均用作抗氧化剂，因此了解植物中的CoQ10生物合成及其代谢工程越来越重要。这项工作审查了各种已知的和假定的生物学功能，CoQ 10在植物中的生物合成和代谢工程。描述了一种用于烟叶中辅酶Q10常规定量的可靠HPLC-UV方法，该方法可以描述在烟叶中，烟草植株的不同茎部位置以及温室中不同时间的辅酶Q10的存在。此外，分别通过农杆菌介导的转化用编码拟南芥4-羟基苯甲酸酯聚异戊二烯二磷酸转移酶的基因转化白肋烟和烤烟的单倍体基因型TN90LC和NC55。鉴定出单独的T0转基因植物具有增加的辅酶Q10含量。由于统计显着的基因型效应，从每个NC55和TN90LC中鉴定出了来自自受精双倍单倍体后代的转基因品系，其CoQ10含量分别增加了60％和40％。辅酶Q10改善的转基因品系显示出比非转基因品更好的从NaCl胁迫中恢复的明显能力。回收的转基因烟草以及非转基因的组织培养烟草也同样被发现具有提高的CoQ10。当通过LC-APCI-MS / MS监测叶绿体衍生的类异戊二烯时，CoQ10增加的转基因DH1 NC55品系在类异戊二烯中没有显示出差异。

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  • 作者

    Stiff, Michael Roy.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Agriculture Plant Culture.;Chemistry Biochemistry.;Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 156 p.
  • 总页数 156
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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