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首页> 外文学位 >Genetic linkage map of creeping bentgrass and characterization of LpCBF3 gene for cold tolerance in perennial ryegrass.
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Genetic linkage map of creeping bentgrass and characterization of LpCBF3 gene for cold tolerance in perennial ryegrass.

机译：多年生黑麦草bent草的遗传连锁图谱和LpCBF3基因耐寒性的表征。

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摘要

The colonial bentgrass (Agrostis capillaris L.) is a potential source of genetic improvement of resistance to environmental stress and disease for other bentgrass species (Agrostis spp.). Genetic diversity in the colonial bentgrass species was investigated using amplified fragment length polymorphism (AFLP) markers. Ten EcoRI/ MseI and six PstI/MseI AFLP primer combinations produced 181 and 128 informative polymorphic bands, respectively. Cluster analysis of genetic similarity estimates revealed a high level of diversity in colonial bentgrass species with averages of 0.51 (EcoR I/MseI) and 0.63 (PstI/ MseI). Greater genetic diversity was detected by the EcoR I/MseI AFLP primer combinations. A low but significant positive correlation (r=0.44, p=0.0099) between the two Jaccard similarity matrices was obtained by the Mantel test.; To improve creeping bentgrass disease resistance, interspecific hybrids between creeping bentgrass and colonial bentgrass were produced for introgression of resistance genes. The hybrids (2n=28) were confirmed by a species-specific sequence characterized amplified region (SCAR) marker of the colonial bentgrass genome. In addition, average seven ring bivalents, several univalents and multivalents were observed at meiosis of hybrids. Pollen fertility of the hybrids ranged from 4% to 34.5%. The hybrids were evaluated for gray snow mold disease resistance in the cold room and dollar spot disease resistance in the greenhouse. Some of hybrids had moderate snow mold and dollar spot resistance, exhibiting partially diminished disease symptoms.; A segregating progeny set of 188 F1 full-sib population was developed by two-way pseudo-testcross mapping strategy for linkage construction and QTL detection in creeping bentgrass. In the male parent map, 93 markers could be grouped in 14 linkages covering a total length of 793cM with an average interval of is 8.2cM, while in the female parent map 139 markers were clustered in other 14 linkages spanning 805cM with an average distance of 5.9cM between adjacent markers. Five putative QTLs were found in this segregation population, three QTLs for leaf width, one QTL for snow mold disease resistance, and one QTL for recovery.; A CBF homologous gene, LpCBF3, was isolated and characterized from a cold-tolerant perennial ryegrass accession (Lolium perenne L.). LpCBF3 encodes a protein of 237 amino acids with a molecular mass of 25.5 kDa. It carries sequences of the typical AP2 DNA-binding domain and an acidic activation which are present in the majority of the plant CBF protein family. Northern blotting and RT-PCR analysis found LpCBF3 reached the highest expression after 90 minutes of cold-treatment (4°C). In Arabidopsis, the over-expression of LpCBF3 under 35S promoter was resulting in plant with dwarfism, later flowering and freezing tolerance. These results lead us to propose potential implications and applications of LpCBF3 gene in turfgrass cold-tolerance and quality breeding program.
机译：结肠草（Agrostis capillaris L.）是其他草种（Agrostis spp。）对环境压力和疾病抗性遗传改良的潜在来源。使用扩增的片段长度多态性（AFLP）标记调查了殖民地草丛中的遗传多样性。十个EcoRI / MseI和六个PstI / MseI AFLP引物组合分别产生181和128个信息多态性条带。遗传相似性估计值的聚类分析显示，结肠弯曲草物种具有很高的多样性，平均值为0.51（EcoR I / MseI）和0.63（PstI / MseI）。 EcoR I / MseI AFLP引物组合检测到更大的遗传多样性。通过Mantel检验获得了两个Jaccard相似度矩阵之间的低但显着正相关（r = 0.44，p = 0.0099）。为了提高creep草的抗病性，生产了bent草和殖民地bent草之间的种间杂种，以使抗性基因渗入。杂种（2n = 28）通过殖民地弯曲草基因组的物种特异性序列特征扩增区域（SCAR）标记得到证实。另外，在杂种的减数分裂中观察到平均七个环二价，几个单价和多价。杂种的花粉育性为4％至34.5％。评价了杂种在冷藏室中对灰雪霉病的抵抗力和在温室中对美元斑病的抵抗力。一些杂种具有中等的雪霉病和美元抗性，表现出部分减轻的疾病症状。通过双向伪-testcross作图策略开发了188个F1全同胞种群的分离后代集，用于在bent草中建立连锁和QTL检测。在雄性父本图中，可以将93个标记分组为14个连锁，覆盖全长793cM，平均间隔为8.2cM，而在雌性父本中，则将139个标记聚集在其他14个连锁中，跨度为805cM，平均距离为相邻标记之间为5.9cM。在该分离种群中发现了五个假定的QTL，三个QTL用于叶宽，一个QTL用于抗雪霉病，一个QTL用于恢复。分离出CBF同源基因LpCBF3，并从耐寒多年生黑麦草（Lolium perenne L.）中鉴定其特征。 LpCBF3编码237个氨基酸的蛋白质，分子量为25.5 kDa。它带有典型的AP2 DNA结合结构域和酸性激活序列，这些序列存在于大多数植物CBF蛋白家族中。 Northern印迹和RT-PCR分析发现，在冷处理90分钟（4℃）后，LpCBF3达到最高表达。在拟南芥中，LpCBF3在35S启动子下的过表达导致植物矮化，开花和耐冻性增强。这些结果使我们提出了LpCBF3基因在草皮耐寒优质育种程序中的潜在意义和应用。

著录项

  • 作者

    Zhao, Han.;

  • 作者单位

    Michigan State University.;

  • 授予单位 Michigan State University.;
  • 学科 Agriculture Agronomy.; Biology Genetics.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 157 p.
  • 总页数 157
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农学（农艺学）;遗传学;
  • 关键词

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