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Genetic diversity and candidate gene selection for drought tolerance in perennial ryegrass.

机译：多年生黑麦草的耐旱性的遗传多样性和候选基因选择。

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摘要

Perennial ryegrass (Lolium perenne L.) is one of the most important cool-season grass species used for forage and turf industries in temperate regions. The improvement of drought tolerance will significantly benefit the sustainable production of perennial ryegrass. The objectives of this study were to: (1) determine the ploidy level and DNA content of global perennial ryegrass germplasm accessions using flow cytometry; (2) analyze genetic diversity in a globally distributed collection of perennial ryegrass accessions with 99 simple sequence repeat (SSR) markers; (3) examine the expression patterns of the candidate genes involved in drought tolerance and responses in both drought-tolerant and -susceptible accessions of perennial ryegrass.;The DNA content and ploidy level of 194 accessions from the USDA National Plant Germplasm System (NPGS) (including the commercial cultivar, Affinity) and six commercial cultivars (Brightstar SLT, Catalina II, Divine, Inspire, Manhattan 4, and Silver Dollar) were determined. Among the 200 germplasm accessions, 194 diploids and 6 tetraploids were identified. The average DNA content was 5.60 pg/2C and 11.45 pg/2C for the diploid and tetraploid, respectively.;A total of 99 simple sequence repeat (SSR) markers were used to examine genetic diversity of 190 germplasm accessions. The cluster analysis using the unweighted pair-group method arithmetic average (UPGMA) showed the genetic diversity of commercial cultivars was limited. The principal component analysis (PCA) indicated that the first two principal components were significant and explained 7.7% and 2.6% of the total variation, respectively.;The transcript levels of HARDY, plasma membrane intrinsic protein (PIP1;4) and ion superoxide dismutase (FeSOD ) were generally higher in one or two drought-tolerant accessions than in the susceptible ones. These candidate genes could be used for marker-trait association mapping of drought tolerance in perennial ryegrass.;Our long-term goal is to establish a natural diploid mapping population and to associate physiological traits with genes responsible for drought tolerance. These three parts of research provide a foundation to achieve the goal.
机译：多年生黑麦草（Lolium perenne L.）是温带地区最重要的凉季草种之一，用于牧草和草皮工业。耐旱性的提高将大大有利于多年生黑麦草的可持续生产。这项研究的目的是：（1）使用流式细胞仪确定全球多年生黑麦草种质材料的倍性水平和DNA含量； （2）分析具有99个简单序列重复（SSR）标记的多年生黑麦草种的全球分布集合中的遗传多样性； （3）研究了多年生黑麦草的耐旱性和易感性材料中与耐旱性有关的候选基因的表达模式和响应;美国农业部国家植物种质系统（NPGS）194种材料的DNA含量和倍性水平（包括商业品种，亲和力）和六个商业品种（Brightstar SLT，Catalina II，Divine，Inspire，Manhattan 4和Silver Dollar）被确定。在200种种质中，鉴定出194个二倍体和6个四倍体。二倍体和四倍体的平均DNA含量分别为5.60 pg / 2C和11.45 pg / 2C。总共使用99个简单序列重复（SSR）标记检查了190个种质的遗传多样性。使用非加权对-群法算术平均值（UPGMA）进行的聚类分析表明，商业品种的遗传多样性有限。主成分分析（PCA）表明前两个主成分很重要，分别解释了总变异的7.7％和2.6％.; HARDY，质膜内在蛋白（PIP1; 4）和离子超氧化物歧化酶的转录水平一或两种耐旱种质中的FeSOD（FeSOD）通常高于易感种质。这些候选基因可用于多年生黑麦草的耐旱性标记-性状关联图谱研究。我们的长期目标是建立天然的二倍体作图种群，并将生理性状与负责耐旱性的基因相关联。这三部分研究为实现该目标提供了基础。

著录项

  • 作者

    Wang, Ying.;

  • 作者单位

    Purdue University.;

  • 授予单位 Purdue University.;
  • 学科 Agriculture Agronomy.
  • 学位 M.S.
  • 年度 2009
  • 页码 103 p.
  • 总页数 103
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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