• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Characterization of the TAF12 and Ada6 components in transcriptional co-activation by the SAGA and SLIK HAT complexes.
【24h】

Characterization of the TAF12 and Ada6 components in transcriptional co-activation by the SAGA and SLIK HAT complexes.

机译：通过SAGA和SLIK HAT复合物在转录共激活中对TAF12和Ada6组分的表征。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Post-translational acetylation of chromatin is a key regulator of transcriptional activation, DNA repair, and genomic replication. The evolutionarily conserved SAGA (Spt-Ada-Gcn5 Acetyltransferase) and SLIK (SAGA- Like) histone acetyltransferase (HAT) complexes are required for transcriptional activation of a subset of yeast genes and contain multiple subunits including the histone fold-containing TBP-Associated Factors (TAFs): 6, 9, 10, and 12. These TAFs are also components of the TFIID complex and are consequently involved in most RNA polymerase II-mediated transcription in yeast. Here we identify a novel conserved domain of TAF12, outside of its histone fold, that is required for SAGA and SLIK-directed nucleosomal acetylation. We demonstrate that this domain is not required for chromatin association, but show that it plays an essential role in histone H3 acetylation at specific SAGA and SLIK-regulated promoters. These data suggest that the ReNu ( Required for Nucleosomal Acetylation) domain of TAF12 regulates Gcn5 acetylation of specific substrates by the SAGA super-family of HAT complexes. Additionally, we characterize a novel subunit, Ada6, shared between SAGA and SLIK. Ada6 mutations exhibit resistance to the toxic chimera GAL4-VP16 and reduced transcriptional capacity. Strikingly, Ada6-deleted SAGA and SLIK are not structurally compromised, but ada6 yeast are inviable on galactose, potassium acetate, and glycerol/ethanol carbon sources. Our data contrasts with reports demonstrating that only deletions of SAGA core structural components are inviable on galactose media. Our data suggest that the Ada6 module of the SAGA and SLIK complexes is required for transcriptional activation of galactose metabolism genes.
机译：染色质的翻译后乙酰化是转录激活，DNA修复和基因组复制的关键调节剂。进化保守的SAGA（Spt-Ada-Gcn5乙酰转移酶）和SLIK（SAGA-like）组蛋白乙酰转移酶（HAT）复合物是酵母基因子集的转录激活所必需的，并且包含多个亚基，包括含组蛋白折叠的TBP相关因子（TAF）：6、9、10和12。这些TAF也是TFIID复合体的组成部分，因此参与了酵母中大多数RNA聚合酶II介导的转录。在这里，我们确定了TAF12的一个新的保守域，在其组蛋白折叠之外，这是SAGA和SLIK指导的核小体乙酰化所必需的。我们证明该域不是染色质缔合所必需的，但表明它在特定的SAGA和SLIK调控的启动子上在组蛋白H3乙酰化中起重要作用。这些数据表明，TAF12的ReNu（核糖体乙酰化必需）域通过HAT复合物的SAGA超家族调节特定底物的Gcn5乙酰化。此外，我们表征了SAGA和SLIK之间共享的新型亚基Ada6。 Ada6突变表现出对有毒嵌合体GAL4-VP16的抗性并降低了转录能力。令人惊讶的是，缺失Ada6的SAGA和SLIK在结构上没有受到损害，但是ada6酵母在半乳糖，乙酸钾和甘油/乙醇碳源上是不可行的。我们的数据与证明半乳糖培养基上只有SAGA核心结构成分缺失的报道形成对比。我们的数据表明，SAGA和SLIK复合物的Ada6模块是半乳糖代谢基因转录激活所必需的。

著录项

  • 作者

    Torok, Michael Scott.;

  • 作者单位

    University of Virginia.;

  • 授予单位 University of Virginia.;
  • 学科 Chemistry Biochemistry.; Biology Molecular.; Biology Genetics.
  • 学位 Ph.D.
  • 年度 2006
  • 页码 365 p.
  • 总页数 365
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;分子遗传学;遗传学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号