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Optimization of the Production of Long-Chain Dicarboxylic Acids from Distillers Corn Oil Using Candida viswanathii

机译：利用黏丝酵母念珠菌从蒸馏玉米油中生产长链二元羧酸的工艺优化

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摘要

This thesis explores the viability of using the yeast Candida viswanathii to convert distillers corn oil, a byproduct of the ethanol industry, into long-chain alpha,o-dicarboxylic acids used in lubricants, cosmetics, and biopolymers. Glucose, xylose, and glycerol were used as carbon sources for the determination of growth parameters of this strain, of which it was found that growth on glucose resulted in the highest specific growth rate of 0.482 hr--1 and the lowest biomass yield coefficient of 0.566--0.754 g DCW per g substrate on average. A prior developed analytical method for determining feed and product concentrations in fermentation broth using gas chromatography was gradually improved throughout this study. However, it was found that repeatability issues still occurred with the method. The production of diacids was studied with different feedstocks and co-substrates, where it was found that diacid production occurred with all combinations, except methyl oleate and glucose. It was observed that both methyl oleate and oleic acid had solubility issues, which could be further improved within the fermentation broth.
机译：本文探讨了使用酵母念珠菌将乙醇工业的副产品蒸馏玉米油转化为用于润滑剂，化妆品和生物聚合物的长链α，o-二羧酸的可行性。葡萄糖，木糖和甘油用作确定该菌株生长参数的碳源，发现在葡萄糖上生长导致最高比生长速率为0.482 hr--1，最低生物量产量系数为每克基材平均0.566--0.754克DCW。在整个研究过程中，逐渐改进了使用气相色谱法测定发酵液中饲料和产物浓度的先前开发的分析方法。但是，发现该方法仍然会发生重复性问题。用不同的原料和共底物研究了二酸的产生，发现除油酸甲酯和葡萄糖外​​，所有组合均产生二酸。观察到油酸甲酯和油酸均具有溶解性问题，这可以在发酵液中进一步改善。

著录项

  • 作者

    Mobley, Jennifer Ann.;

  • 作者单位

    Rose Hulman Institute of Technology.;

  • 授予单位 Rose Hulman Institute of Technology.;
  • 学科 Chemical engineering.;Bioengineering.
  • 学位 M.S.
  • 年度 2018
  • 页码 147 p.
  • 总页数 147
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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