• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >The application of a passive dosing system for determining zebrafish early life stage toxicity of hydrocarbons for use in calibrating a predictive model to acute and chronic endpoints.
【24h】

The application of a passive dosing system for determining zebrafish early life stage toxicity of hydrocarbons for use in calibrating a predictive model to acute and chronic endpoints.

机译：被动加药系统在确定斑马鱼碳氢化合物生命早期毒性方面的应用，可用于校准对急性和慢性终点的预测模型。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The target lipid model has been used to derive water quality objectives that are intended to be protective of chronic effects posed by hydrocarbons. However, experimental Early Life Stage toxicity data for fish are limited and further data are needed to confirm model predictions. Efforts are underway to develop a zebrafish embryo toxicity test guideline to reduce, refine and replace the use of vertebrates in animal testing. An adaptation of this method which includes embryo lethal and sub-lethal developmental endpoints after a 5 day exposure as well as larval survival and growth endpoints during a subsequent 25 day test period is described. To deliver well controlled exposure concentrations, a passive dosing system consisting of silicone coated vials and silicone O-rings was employed. This research consisted of three phases. During the first phase, phenanthrene was used as a reference substance to test efficacy of a passive dosing design as well as compare observed effect data with published effect data. Concentrations shown to cause adverse effects in this phase of the research were in the range of previous studies that have investigated the chronic effects of phenanthrene on fish. In the second phase, separate exposures to five aromatic hydrocarbons (AHs) including 1-methylnaphthalene, phenanthrene, 1, 2, 3, 4, 5, 6, 7, 8, octahydrophenanthrene, benzo(a)pyrene and chrysene utilizing the passive dosing system and ELS design were investigated. Subsequently, during phase two, six short-term (120 hour) exposures for naphthalene, 1-methylnaphthalene, biphenyl, phenanthrene, pyrene and 1, 2, 3, 4, 5, 6, 7, 8, - octahydrophenanthrene were also employed. Data collected from acute and chronic exposures were used to estimate the acute and chronic critical target lipid body burdens for zebrafish using the TLM framework. During the third phase a simple mixture of ten saturated, unsaturated, and aromatic hydrocarbons with a KOW range of ca. 4 - 7.5 was tested during a 30 day ELS test. Three treatment levels were selected to provide concentrations that corresponded to a toxic unit range above and below the predicted effect concentration assuming concentration addition. Observed acute and chronic effects with the mixture were consistent with the assumption of additive toxicity.
机译：目标脂质模型已用于得出水质目标，旨在保护碳氢化合物造成的慢性影响。但是，鱼类的实验性早期生命毒性数据有限，需要进一步的数据来确认模型预测。正在努力制定斑马鱼胚胎毒性测试指南，以减少，完善和替代脊椎动物在动物测试中的使用。描述了该方法的一种改编，包括暴露5天后的胚胎致死和亚致死发育终点，以及随后的25天测试期间的幼虫存活和生长终点。为了提供良好控制的暴露浓度，采用了由硅酮涂层的样品瓶和硅酮O形圈组成的被动定量系统。这项研究包括三个阶段。在第一阶段，将菲用作参考物质，以测试被动剂量设计的功效，并将观察到的效果数据与已发布的效果数据进行比较。在该研究阶段显示出不利影响的浓度在以前的研究中，该研究调查了菲对鱼类的慢性影响。在第二阶段中，通过被动加料分别暴露于五个芳香族烃（AH），包括1-甲基萘，菲，1、2、3、4、5、6、7、8，八氢菲，苯并（a）py和系统和ELS设计进行了调查。随后，在第二阶段中，还对萘，1-甲基萘，联苯，菲，pyr和1、2、3、4、5、6、7、8，-八氢菲进行了六次短期（120小时）暴露。从急性和慢性暴露中收集的数据用于使用TLM框架估算斑马鱼的急性和慢性关键目标脂质体负担。在第三阶段中，十种饱和，不饱和和芳烃的简单混合物，其KOW范围约为。在30天的ELS测试中测试了4-7.5。选择三种处理水平以提供对应于假设添加浓度的，高于和低于预期效果浓度的毒性单位范围的浓度。观察到的混合物的急性和慢性作用与假定的附加毒性相符。

著录项

  • 作者

    Butler, Joshua D.;

  • 作者单位

    Rutgers The State University of New Jersey - New Brunswick.;

  • 授予单位 Rutgers The State University of New Jersey - New Brunswick.;
  • 学科 Environmental health.;Water resources management.
  • 学位 Ph.D.
  • 年度 2013
  • 页码 155 p.
  • 总页数 155
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号