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首页> 外文学位 >Rapid Determination of Aflatoxin B1, B2, G1, and G2 in Corn Meal Using Disposable Pipette Extraction (DPX) Followed By High Performance Liquid Chromatography (HPLC) Analysis.
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Rapid Determination of Aflatoxin B1, B2, G1, and G2 in Corn Meal Using Disposable Pipette Extraction (DPX) Followed By High Performance Liquid Chromatography (HPLC) Analysis.

机译：使用一次性移液器萃取（DPX）快速测定玉米粉中黄曲霉毒素B1，B2，G1和G2的含量，然后进行高效液相色谱（HPLC）分析。

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摘要

Aflatoxins B1, B2, G1, and G2 (AFB1, AFB2, AFG1, AFG2) are toxic metabolites generated by Aspergillus flavus and Aspergillus parasiticus. These toxins have been reported to be associated with acute liver damage, liver cirrhosis, induction of tumors and teratogenic effects. The presence of Aaflatoxins in many agricultural products such as corn, nuts, grain, and spices has been a global concern by both regulatory departments and the public. Therefore it is important to develop a fast sample preparation method for aflatoxins analysis in foods in response to food emergency.;Traditional immunoassay and solid-phase extraction (SPE) techniques have been used for extraction of aflatoxins in foods; however, those methods usually involve tedious conditioning steps and evaporation of large amounts of organic solvents. Disposable pipette extraction (DPX) is a type of newly developed SPE method that is easy, quick, and reliable. DPX eliminates the use of large volumes of organic solvents and is environmentally friendly. The current research employed sample preparation using DPX extraction followed by high performance liquid chromatography (HPLC) analysis of Aflatoxins B1, B2, G1, and G2. Mean recoveries for all targeted aflatoxins were determined to be above 80% with relative standard deviation of 13.3%, which showed good accuracy and precision. A matrix match calibration study was done using a cornmeal sample containing the aflatoxins to evaluate the linearity, limit of detection (LOD), limit of quantification (LOQ) of the current method. Good linearity was obtained for all aflatoxins with R2 value greater than 0.993. LOD and LOQ were found to be below 6.3 ppb and 19.01 ppb, respectively. After method validation, this technique was applied to real samples of corn muffin mix. It was found that the SPE technique using DPX is rapid, simple, and accurate for monitoring aflatoxins in corn products.
机译：黄曲霉毒素B1，B2，G1和G2（AFB1，AFB2，AFG1，AFG2）是由黄曲霉和寄生曲霉产生的有毒代谢产物。据报道，这些毒素与急性肝损伤，肝硬化，诱导肿瘤和致畸作用有关。黄曲霉毒素在许多农产品中的存在，例如玉米，坚果，谷物和香料，一直是监管部门和公众关注的全球问题。因此，开发一种快速响应食品紧急情况的食品中黄曲霉毒素分析样品制备方法非常重要。传统的免疫分析和固相萃取（SPE）技术已用于食品中黄曲霉毒素的提取；然而，这些方法通常涉及繁琐的调节步骤和大量有机溶剂的蒸发。一次性移液器萃取（DPX）是一种新开发的SPE方法，该方法简便，快速且可靠。 DPX无需使用大量有机溶剂，并且对环境无害。当前的研究采用的样品制备方法是先用DPX提取，再用高效液相色谱（HPLC）分析黄曲霉毒素B1，B2，G1和G2。经测定，所有目标黄曲霉毒素的平均回收率均在80％以上，相对标准偏差为13.3％，显示出良好的准确性和精密度。使用含有黄曲霉毒素的玉米面样品进行基质匹配校准研究，以评估当前方法的线性，检测限（LOD）和定量限（LOQ）。所有R2值均大于0.993的黄曲霉毒素均具有良好的线性。发现LOD和LOQ分别低于6.3 ppb和19.01 ppb。在方法验证之后，该技术被应用于玉米松饼混合物的真实样品。已经发现，使用DPX的SPE技术可以快速，简单和准确地监测玉米产品中的黄曲霉毒素。

著录项

  • 作者

    Fitzgerald, Samantha.;

  • 作者单位

    Western Illinois University.;

  • 授予单位 Western Illinois University.;
  • 学科 Agriculture Food Science and Technology.;Chemistry Analytical.
  • 学位 M.S.
  • 年度 2013
  • 页码 117 p.
  • 总页数 117
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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