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首页> 外文期刊>Journal of the Brazilian Chemical Society >Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection
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Rapid and Effective Sample Cleanup based on Polyvinylpyrrolidone-Coated Magnetite Nanoparticles Coupled with New Pre-Column Derivatization for Determination of Aflatoxins B1, B2, G1 and G2 in Animal Feeds by High-Performance Liquid Chromatography with Fluorescence Detection

机译：基于聚乙烯基吡咯烷酮涂覆的磁铁矿纳米粒子的快速且有效的样品清洁，通过具有高性能液相色谱法测定动物饲料中的新型柱状衍生化，通过高性能液相色谱法测定荧光检测

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摘要

A novel method for the determination of aflatoxins B1, B2, G1, and G2 (AFB1, AFB2, AFG1, AFG2) in animal feed samples based on magnetic solid-phase cleanup and novel pre-column derivatization has been developed. In this work, synthesized polyvinylpyrrolidone-coated Fe3O4 magnetic nanoparticles were applied as adsorbents for rapid cleanup for extracts of animal feed samples. The purified extract was then derivatized with trifluoroacetic acid coupling ultraviolet irradiation which enabled the weakly fluorescent AFB1 and AFG1 transformed into their highly fluorescent hemiacetals derivatives. Afterward aflatoxins were separated and quantified by reverse phase liquid chromatography with fluorescence detection. The results indicated that four AFs presented excellent linear response with correlation coefficients 0.996 and recoveries ranged from 86.7 to 108.9% with relative standard deviations (RSDs) from 3.6 to 6.4%. The limits of detection (LODs) were 0.0392, 0.2429, 0.0842, 0.4556 ng mL-1 for AFB1, AFB2, AFG1 and AFG2, respectively.
机译：已经开发了一种新的用于基于磁共相清除和新型预衍生物的动物进料样品中的用于测定用于测定用于基于磁共相清除和新型柱前衍生化的新型毒素B1，B2，G1和G2（AFB1，AFB2，AFG1，AFG2）。在这项工作中，合成的聚乙烯吡咯烷酮涂覆的Fe3O4磁性纳米颗粒作为吸附剂施用，用于快速清理动物进料样品的提取物。然后用三氟乙酸偶联紫外线辐射衍生纯化的提取物，使得使弱荧光的AFB1和AFG1转化为其高荧光半导体衍生物。通过反相液相色谱法分离并定量随后的黄曲霉毒素，并通过荧光检测进行定量。结果表明，四个AFS呈现出优异的线性响应，相关系数> 0.996，回收率范围为86.7至108.9％，相对标准偏差（RSD）从3.6升至6.4％。检测极限（LOD）为0.0392,0.2429,0.0842,0.0842，分别用于AFB1，AFB2，AFG1和AFG2的0.4556ng ML-1。

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