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首页> 外文学位 >The role of testicular germ cell apoptosis during equine spermatogenesis .
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The role of testicular germ cell apoptosis during equine spermatogenesis .

机译：睾丸生殖细胞凋亡在马精子发生中的作用。

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摘要

Apoptosis in testicular germ cells has been demonstrated in many species. Features of apoptosis reported in other species were used to confirm use of the TUNEL assay in stallion testes. Eight stallions with normal testicular size and semen quality were evaluated to determine the germ cell types and stages where apoptosis most commonly occurs. Mean numbers of TUNEL-positive germ cells per 100 Sertoli cell nuclei were highest in stages IV and V of the seminiferous epithelial cycle corresponding to meiotic divisions of primary spermatocytes and mitotic proliferation of B1 and B2 spermatogonia. Round and elongated spermatids were labeled less frequently by the TUNEL assay. To examine the relationships between germ cell apoptotic rate and spermatogenic efficiency, seminal traits were assessed to classify stallions into normal or reduced quality semen groups. Apoptotic rates were higher for stages IV-VI and stage VIII seminiferous tubules in stallions with reduced semen quality. Daily sperm production (DSP) per gram and per testis were lower for stallions with reduced semen quality. Regression analyses revealed negative linear relationships for germ cell apoptotic rate with DSP/g, DSP/testis, daily sperm output, progressively motile sperm and morphologically normal sperm in ejaculates. Mean circulating concentrations of inhibin were lower for stallions ejaculating reduced quality semen. Apoptotic rate was negatively correlated with concentrations of inhibin and estradiol-17β and positively correlated with concentrations of LH and FSH.;To study germ cell apoptosis and formation of the Sertoli cell barrier during the initiation of spermatogenesis, tubule development was classified based on lumen score. Formation of a seminiferous tubule lumen was consistent with events leading to development of a Sertoli cell barrier. A primary wave of apoptosis removed early differentiating germ cell types prior to the formation of a tubule lumen facilitating both the formation of a tubule lumen and a Sertoli cell barrier. A second wave of apoptosis occurred after the formation of a lumen but before seminiferous tubule cross-sections contained a full complement of germ cells.;In conclusion, apoptosis is an essential mechanism during normal spermatogenesis. Apoptosis also accounts for low numbers of normal sperm seen in the ejaculates of some stallions.
机译：睾丸生殖细胞的凋亡已在许多物种中得到证实。在其他物种中报道的凋亡特征被用于确认在种马睾丸中使用TUNEL分析。评价了八种具有正常睾丸大小和精液质量的种马，以确定最常发生凋亡的生殖细胞类型和阶段。每100个支持细胞核的TUNEL阳性生殖细胞平均数最高的是生精上皮周期的IV和V期，对应于原代精母细胞的减数分裂以及B1和B2精原细胞的有丝分裂增殖。圆形和细长的精子细胞通过TUNEL分析的频率较低。为了检查生殖细胞凋亡率与生精效率之间的关系，评估了精子性状以将种马分为正常或质量下降的精液组。在精液质量降低的种马中，IV-VI期和VIII期曲细精管的细胞凋亡率更高。对于精液质量下降的种马，每克和每个睾丸的每日精子产量（DSP）较低。回归分析显示，精液中生殖细胞凋亡率与DSP / g，DSP /睾丸，每日精子输出，逐渐活动的精子和形态正常的精子呈负线性关系。对于射精质量下降的种马，抑制素的平均循环浓度较低。凋亡率与抑制素和雌二醇-17β的浓度呈负相关，与LH和FSH的浓度呈正相关。为了研究精子发生过程中生殖细胞的凋亡和Sertoli细胞屏障的形成，根据管腔分数对小管发育进行了分类。 。曲细精管腔的形成与导致支持细胞屏障形成的事件一致。凋亡的初级波在形成小管腔之前消除了早期分化的生殖细胞类型，从而促进了小管腔的形成和支持细胞的屏障。凋亡的第二波发生在管腔形成后，但在生精小管横截面包含完整的生殖细胞之前。；总而言之，凋亡是正常精子发生过程中的重要机制。凋亡还导致某些种马的射精中正常精子数量少。

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  • 作者

    Heninger, Noah Leland, III.;

  • 作者单位

    Texas A&M University.;

  • 授予单位 Texas A&M University.;
  • 学科 Biology Animal Physiology.;Biology Veterinary Science.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 135 p.
  • 总页数 135
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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