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首页> 外文学位 >Explore the murine cardiac 20S proteasomes: Molecular composition and regulation.
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Explore the murine cardiac 20S proteasomes: Molecular composition and regulation.

机译：探索鼠心脏20S蛋白酶体：分子组成和调控。

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摘要

20S proteasome, essential component of protein degradation mechanism, is important to maintain homeostasis. Its malfunctions have been associated with several pathological conditions. This study presents an extensive study of murine cardiac 20S proteasome. Using biochemical methods, 20S proteasome have been purified to 95%. Proteomic study identified all 20S proteasome subunits. Endogenous phosphorylation was also documented. Furthermore, several associating kinases and phosphatase were identified. They regulated its activities. In PKCepsilon over-expression mice, 20S proteasome expression level was up-regulated, but its peptidase activities did not increase. alphaB crystallin were recruited to PKCepsilon subproteome in the transgenic mice, which also associated with 20S proteasome. This association was enhanced in the transgenic mice and has been reported to inhibit 20S proteasome activities. It suggested alphaB crystallin play a role in cardiac 20S proteasome regulation.
机译：20S蛋白酶体是蛋白质降解机制的重要组成部分，对于维持体内平衡非常重要。其故障与几种病理状况有关。这项研究提出了小鼠心脏20S蛋白酶体的广泛研究。使用生化方法，已将20S蛋白酶体纯化至95％。蛋白质组学研究确定了所有20S蛋白酶体亚基。还记录了内源性磷酸化。此外，鉴定了几种相关的激酶和磷酸酶。他们规范了它的活动。在PKCepsilon过表达的小鼠中，20S蛋白酶体的表达水平上调，但其肽酶活性并未增加。在转基因小鼠中也将alphaB crystallin募集到PKCepsilon子蛋白质组中，该蛋白也与20S蛋白酶体相关。这种关联在转基因小鼠中得到了增强，并且据报道抑制了20S蛋白酶体的活性。这表明αB晶状体蛋白在心脏20S蛋白酶体调节中起作用。

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  • 作者

    Zong, Chenggong.;

  • 作者单位

    University of Louisville.;

  • 授予单位 University of Louisville.;
  • 学科 Molecular biology.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 132 p.
  • 总页数 132
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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