• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >A metabolomic study of alfalfa plants genetically engineered to overproduce a key enzyme in nitrogen metabolism, the cytosolic form of glutamine synthetase.
【24h】

A metabolomic study of alfalfa plants genetically engineered to overproduce a key enzyme in nitrogen metabolism, the cytosolic form of glutamine synthetase.

机译：紫花苜蓿植物的代谢组学研究，其经过基因工程处理以过量产生氮代谢中的关键酶，即谷氨酰胺合成酶的胞质形式。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study we have conclusively demonstrated that increasing GS1 activity using the CaMV 35S promoter to drive the soybean GS 1 gene (Gmglnb1) without its 3'UTR in alfalfa is accompanied by increase in GS activity and produces unique phenotypic, physiological, and metabolic features different from control plants. Differences include long internodes, early flowering and a small increase in biomass. At the physiological level, we see an increase in chlorophyll content but little increase in photosynthetic rates. The transformants showed an increase in total amino acids and protein content accounting for improved nitrogen use efficiency. There was no change in the elemental carbon content and in fact a drop in starch levels.;Analysis of the polar metabolite extract of the leaves of control and transgenic plants by GC/MS, showed a definite change in metabolite profile between the two classes of plants. The majority of carbohydrates, phosphate sugars, and organic acids detected in this study showed a decrease in concentration in the transgenic plants. All the evidence indicates that the unregulated expression of GS1 in alfalfa resulting in increased GS1 activity causes a reduction in carbon containing metabolites and may be the cause for limited increase in plant biomass. To elucidate altered biochemical networks in transformants, we looked at metabolite correlation matrices and their network topology.;We propose from the evidence found in this work that although GS 1 overexpressers have a positive impact on NUE, any further improvement in NUE along with increase in biomass can be attained by co-expressing GS 1 with a gene for a key enzyme in sucrose synthesis. One such enzyme sucrose phosphate synthase (SPS), increases the synthesis of carbon skeletons and substrates for energy production.
机译：在这项研究中，我们最终证明了使用CaMV 35S启动子来驱动大豆GS 1 基因（ Gmglnb1 ）时，增加GS 1 活性的方法苜蓿中的3'UTR伴随着GS活性的增加，并产生与对照植物不同的独特表型，生理和代谢特征。差异包括节间长，早开花和生物量少量增加。在生理学水平上，我们看到叶绿素含量增加，但光合速率几乎没有增加。转化子显示出总氨基酸和蛋白质含量的增加，这解释了提高的氮利用效率。元素碳含量没有变化，但实际上淀粉含量却下降了；通过GC / MS对对照和转基因植物叶片的极性代谢物提取物进行分析，结果显示两类代谢物之间的代谢物特征发生了明显变化。植物。在这项研究中检测到的大多数碳水化合物，磷酸糖和有机酸显示出转基因植物中浓度的降低。所有证据表明，苜蓿中GS 1 的失控表达导致GS 1 活性增加，导致含碳代谢产物减少，可能是植物生长受限的原因。生物质。为了阐明转化子中生化网络的变化，我们研究了代谢物相关矩阵及其网络拓扑。我们从这项工作中发现的证据提出，尽管GS 1 过表达子对NUE有积极影响， GS 1 与蔗糖合成中关键酶的基因共表达可实现NUE的改善以及生物量的增加。一种这样的酶蔗糖磷酸合酶（SPS）增加了碳骨架和底物的合成以产生能量。

著录项

  • 作者

    Holguin, F. Omar.;

  • 作者单位

    New Mexico State University.;

  • 授予单位 New Mexico State University.;
  • 学科 Agriculture Agronomy.;Agriculture Plant Culture.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 139 p.
  • 总页数 139
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号