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首页> 外文学位 >Genetic and molecular analyses of factors defining host-specificity and virulence in Striga species (Scrophulariaceae).
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Genetic and molecular analyses of factors defining host-specificity and virulence in Striga species (Scrophulariaceae).

机译：遗传和分子分析，定义了Striga物种（玄参科）宿主特异性和毒力的因素。

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摘要

The genus Striga is composed of about 50 species, all obligate parasites of tropical cereals and legumes. The genetic structure of S. asiatica of Benin and S. gesnerioides (SG) were analyzed using AFLP. In the former, the mean within population genetic distances ranged from 0.028 to 0.038, while the mean among-population genetic distances ranged from 0.019 to 0.088, with an assumed minimum genetic distance of 0.01 in each case. The intra- and inter-population variation was reflected by a highly significant R2 value of 0.61 (Mantel's test). Analysis of SG from across West Africa parasitic on cowpea (Vigna unguiculata ) and a strain from Central Florida (SGFL) parasitic on Indigofera hirsuta showed that isolates of this parasite are very uniform genetically. However, there were sufficient differences among the different races of the parasite from West Africa and the strain from Florida that allowed for the identification of molecular markers that were specific to each category using specific EcoRI and MseI selective primer combinations. A phenetic relationship among the different races of S. gesnerioides from West Africa showed that SG race 1 (SG1) and SG5 were most closely related, while SG4 and SG3 were most diverged. A race of the parasite not previously characterized was recognized from Senegal. Although the data seem to suggest that geographic isolation contributes to race formation in this species, host-driven selection is obviously the most critical factor defining race and host-specificity in SG . This species is capable of transgressing hosts. The development of attached SGFL seedlings on cowpea roots were arrested after tubercle formation, while all collections of SGFL failed to parasitize cowpea genotypes known to be susceptible to all cowpea- SG races from West Africa, although root exudates from these cowpea genotypes stimulated the germination of SGFL.; mRNA differential display using total RNA from tubercles of different sizes for two classes of compatible interactions and one class of incompatible interaction of SG with its host were evaluated and differentially expressed cDNA profiles were cloned and sequenced. One cDNA clone from each class of the compatible interaction showed a significant match with ubiquitin-specific proteases, while a clone from an incompatible interaction showed a significant match with organic cation transporter. The cDNA differential display profiles were identical for all sizes of tubercles within each category, suggesting that genes are constitutively expressed in SG tubercles. (Abstract shortened by UMI.)
机译：斯特里加属约50种，全部为热带谷物和豆类的专性寄生虫。贝宁和S. gersnerioides（SG）的遗传结构分析使用AFLP。在前者中，群体内遗传距离的平均值介于0.028至0.038之间，而种群间遗传距离的平均值介于0.019至0.088之间，假定每种情况下的最小遗传距离均为0.01。 R2值为0.61（Mantel检验），反映了种群内和种群间的差异。对来自整个西非cow豆（Vigna unguiculata）寄生虫和来自佛罗里达中部（SGFL）寄生于印度靛蓝（Indigofera hirsuta）的菌株进行的SG分析表明，这种寄生虫的分离物在遗传上非常一致。但是，来自西非的寄生虫和来自佛罗里达的菌株的不同种族之间存在足够的差异，这允许使用特定的EcoRI和MseI选择性引物组合鉴定针对每个类别的特异性分子标记。来自西非的非洲格氏链球菌的不同种族之间的物候关系表明，SG种族1（SG1）和SG5关系最密切，而SG4和SG3分歧最大。塞内加尔认识到以前没有特征的寄生虫种族。尽管数据似乎表明地理隔离有助于该物种的种族形成，但宿主驱动的选择显然是定义SG中种族和宿主特异性的最关键因素。该物种能够侵害宿主。结节形成后，cow豆根上附着的SGFL幼苗的发育被阻止，而所有SGFL的收集都未能使已知对西非所有cow豆SG种族敏感的cow豆基因型寄生，尽管这些cow豆基因型的根系分泌物刺激了玉米的萌发。 SGFL。使用来自不同大小结节的总RNA进行SG与宿主的两类兼容相互作用和一类不兼容相互作用的mRNA差异显示，并克隆和测序差异表达的cDNA图谱。来自每一类兼容相互作用的cDNA克隆显示与泛素特异性蛋白酶显着匹配，而来自不兼容相互作用的克隆显示与有机阳离子转运蛋白显着匹配。每个类别中所有大小结核的cDNA差异显示图谱都相同，这表明基因在SG结核中组成性表达。 （摘要由UMI缩短。）

著录项

  • 作者

    Botanga, Christopher Jomia.;

  • 作者单位

    University of Virginia.;

  • 授予单位 University of Virginia.;
  • 学科 Agriculture Plant Pathology.; Biology Molecular.; Biology Genetics.
  • 学位 Ph.D.
  • 年度 2005
  • 页码 215 p.
  • 总页数 215
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物病理学;分子遗传学;遗传学;
  • 关键词

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