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Development of Electrochemical Sensors for Biodegradable Metallic Implants and Development of a Label-free Biosensor for Bacteria.

机译：用于可生物降解的金属植入物的电化学传感器的开发和用于细菌的无标签生物传感器的开发。

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摘要

This dissertation describes development and characterization of the electrochemical sensors for studying the corrosion of biodegradable metallic implant for the overall RMB research project and electrochemical biosensors for the detection of bacteria. Specifically, three major projects were done. The first involved fabrication and characterization of electrochemical sensors for the cations Zn2+, Ca2+, Mg2+, and H + using carbon nanotubes (CNTs). Detection of Zn2+ was based on an ASV sensor at a CNT tower electrode with a detection limit of 67 nM without any toxic additives. Sensors for the cations Ca2+ , Mg2+, and H+ were solid contact-ion selective electrode (SC-ISE) based on CNT towers. SC-ISE can detect Ca 2+ as low as 1.6 microM with a stable potential for up to three weeks. These electrodes can detect other cations by changing the sensing membrane.;The second part was the development of three systems to study the corrosion of biodegradable metallic implants: corrosion characterization system (CCS), static in vitro CCS, and dynamic in vitro CCS. Multiple appropriate sensors were chosen and applied to these three systems to monitor the degradation of Mg alloy simultaneously and continuously.;The third part was the development of label-free biosensor for bacteria based on faradaic EIS as a spin-off project of RMB. alpha-Mannoside was immobilized on a gold disk electrode using a SAM via a spacer terminated in a thiol functionality. A linear relationship between normalized Ret and the logarithmic value of E. coli concentrations was found in a range of bacterial concentration from 102 to 103 CFU/mL. The combination of robust carbohydrate ligands with EIS provides a label-free, sensitive, specific, user-friendly, robust, and portable biosensing system that could potentially be used in point-of-care or continuous environmental monitoring settings.;The development of electrochemical sensors based on advanced carbon materials and the development of three testing systems (CCS, static in vitro CCS and dynamic in vitro CCS) show new testing methods for biodegradable metallic implants, especially for Mg alloys. The preliminary results presented in this dissertation show that it is possible to monitor corrosion of Mg alloys using two or three electrochemical sensors simultaneously. In the future, the integration of more electrochemical sensors including the developed sensors in Chapter 2 and Chapter 3 will provide more information about the corrosion. With this purpose, the developed electrochemical sensors in Chapter 2 and Chapter 3 should be studied more with respect to selectivity and biofouling in biological applications.
机译：本文介绍了用于RMB研究项目的电化学传感器的开发与表征，以及用于细菌检测的电化学生物传感器的研究和表征。具体来说，完成了三个主要项目。首先涉及使用碳纳米管（CNT）制造和表征用于阳离子Zn2 +，Ca2 +，Mg2 +和H +的电化学传感器。 Zn2 +的检测基于CNT塔电极上的ASV传感器，检测限为67 nM，无任何有毒添加剂。阳离子Ca2 +，Mg2 +和H +的传感器是基于CNT塔的固体接触离子选择电极（SC-ISE）。 SC-ISE可以检测到低至1.6 microM的Ca 2+，并具有长达三周的稳定潜力。这些电极可以通过改变传感膜来检测其他阳离子。第二部分是研究可生物降解金属植入物腐蚀的三种系统的开发：腐蚀表征系统（CCS），体外静态CCS和动态体外CCS。选择了多个合适的传感器并将其应用到这三个系统中，以同时连续地监测镁合金的降解。第三部分是基于法拉第EIS的无标签细菌生物传感器的开发，这是人民币的一个衍生项目。使用SAM通过端基为硫醇官能团的间隔基将α-甘露糖苷固定在金盘电极上。在102至103 CFU / mL的细菌浓度范围内，发现归一化Ret与大肠杆菌浓度的对数值之间存在线性关系。强大的碳水化合物配体与EIS的结合提供了无标签，灵敏，特异，用户友好，强大且便携式的生物传感系统，可潜在地用于即时护理或连续环境监测环境。基于先进碳材料的传感器和三种测试系统（CCS，静态体外CCS和动态体外CCS）的开发展示了可生物降解金属植入物（尤其是镁合金）的新测试方法。本文提出的初步结果表明，可以同时使用两个或三个电化学传感器来监测镁合金的腐蚀。将来，集成更多的电化学传感器（包括第2章和第3章中开发的传感器）将提供有关腐蚀的更多信息。为此，应该在生物应用中就选择性和生物污染方面对第2章和第3章中开发的电化学传感器进行更多研究。

著录项

  • 作者

    Guo, Xuefei.;

  • 作者单位

    University of Cincinnati.;

  • 授予单位 University of Cincinnati.;
  • 学科 Inorganic chemistry.;Materials science.;Biomedical engineering.
  • 学位 Ph.D.
  • 年度 2012
  • 页码 164 p.
  • 总页数 164
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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