• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Determination of lactose by reversed-phase high-performance liquid chromatography.
【24h】

Determination of lactose by reversed-phase high-performance liquid chromatography.

机译：反相高效液相色谱法测定乳糖。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300--360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area to lactose concentration of 0.018 mg/mL to 0.36 mg/mL was obtained with an estimated detection limit of 0.0036 mg/mL lactose concentration. The reproducibility was established with a 4.95% relative standard deviation. The average recovery was 107%, and results were in strong agreement with the standard alkaline ferricyanide method, establishing accuracy.
机译：乳糖是乳制品中常见的二糖。然而，对于乳糖不耐症的人来说，它可能会引起不适。因此，需要准确，可靠和有效地确定食物中的乳糖含量。这项研究的目的是开发一种具有紫外检测的反相HPLC方法来测定乳糖。氨基苯甲酸被用来制备衍生物，使得在300--360 nm范围内检测成为可能。固定相为C8色谱柱，以pH值为5的85％甲醇和15％水混合物作为流动相。获得的峰面积对乳糖浓度为0.018 mg / mL到0.36 mg / mL的线性响应，估计检出限为0.0036 mg / mL乳糖浓度。建立了可重复性，相对标准偏差为4.95％。平均回收率为107％，结果与标准碱性铁氰化物方法高度吻合，建立了准确度。

著录项

  • 作者

    Sexton, Danessa.;

  • 作者单位

    East Tennessee State University.;

  • 授予单位 East Tennessee State University.;
  • 学科 Chemistry Analytical.
  • 学位 M.S.
  • 年度 2004
  • 页码 94 p.
  • 总页数 94
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号