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首页> 外文期刊>Journal of AOAC International >Simultaneous determination of cefixime trihydrate and dicloxacillin sodium in pharmaceutical dosage form by reversed-phase high-performance liquid chromatography.
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Simultaneous determination of cefixime trihydrate and dicloxacillin sodium in pharmaceutical dosage form by reversed-phase high-performance liquid chromatography.

机译:反相高效液相色谱法同时测定药物剂型中的头孢克肟三水合物和双氯西林钠。

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摘要

A simple, precise, accurate, and sensitive RP-HPLC method for simultaneous determination of cefixime trihydrate and dicloxacillin sodium in combined tablet dosage form was developed and validated. Chromatographic separation of the two drugs was performed on a Purospher BDS C18 column (25 cm x 4.6 mm id, 5 microm particle size). The mobile phase methanol-0.01 M phosphate buffer (75 + 25, v/v), adjusted to pH 3 with glacial acetic acid, was delivered at a flow rate of 1.0 mL/min. Detection was performed at 227 nm. Separation was completed within 10 min. Calibration curves were linear with R2 between 0.99 to 1.0 over a concentration range of 2-10 microg/mL for cefixime trihydrate and 5-25 micromL for dicloxacillin sodium. The RSD for intraday and interday precision was < 2.0%.
机译:建立并验证了同时测定头孢克肟三水合物和双氯西林钠组合片剂剂型的简单,精确,准确和灵敏的RP-HPLC方法。两种药物在Purospher BDS C18色谱柱(25 cm x 4.6 mm内径,5微米粒径)上进行色谱分离。用冰醋酸将pH调节至pH 3的流动相甲醇-0.01 M磷酸盐缓冲液(75 + 25,v / v)以1.0 mL / min的流速输送。在227nm进行检测。分离在10分钟内完成。校准曲线是线性的,R2浓度在0.99至1.0之间,三水合头孢克肟的浓度范围为2-10微克/毫升,双氯西林钠的浓度范围为5-25微毫升。日内和日间精度的RSD <2.0%。

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