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Functional analyses of soybean (Glycine max) group 1 and 2 late embryogenesis abundant (LEA) proteins.

机译:大豆(Glycine max)第1组和第2组晚胚发生丰富(LEA)蛋白的功能分析。

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摘要

Late embryogenesis abundant (LEA) proteins are ubiquitous in plants and accumulate during the later stages of seed maturation and in vegetative tissues following dehydration, osmotic, salinity, and low-temperature stress or ABA application. Many LEA proteins also belong to a group of proteins called 'hydrophilins' due to their high hydrophilicity and glycine residue content, high degree of evolutionary conservation, and expression in response to water-deficit stress. Certain classes of LEA proteins have been demonstrated to improve cold, osmotic, salinity, and water deficit tolerance in yeast and plants, however, the exact function of most LEA proteins remains unclear.; To test the functional role of group 1 and group 2 LEA proteins from soybean in stress adaptation, we constitutively expressed the soybean group 1 LEA protein, GmD-19, and the soybean group 2 LEA protein, GmDHN1, respectively, in transgenic Arabidopsis thaliana . Results suggest that group 1 and group 2 LEA proteins can confer protection against salinity, and water-deficit stress and group 2 LEA proteins can confer resistance to freezing stress. Possible mechanisms for the observed improvements in abiotic stress resistance are discussed. (Abstract shortened by UMI.)
机译:脱水,渗透,盐度和低温胁迫或ABA施用后,晚期胚发生富集(LEA)蛋白在植物中普遍存在,并在种子成熟的后期和营养组织中积累。许多LEA蛋白由于它们的高亲水性和甘氨酸残基含量,高度的进化保守性以及响应缺水胁迫的表达,也属于称为“亲水蛋白”的一组蛋白质。已经证明某些类型的LEA蛋白可以改善酵母和植物的耐寒,渗透压,盐度和水分缺乏耐受性,但是,大多数LEA蛋白的确切功能仍不清楚。为了测试大豆中第1组和第2组LEA蛋白在逆境适应中的功能作用,我们在转基因拟南芥中分别表达了大豆第1组LEA蛋白GmD-19和大豆第2组LEA蛋白GmDHN1。结果表明,第1组和第2组LEA蛋白可以赋予抗盐碱保护作用,缺水胁迫和第2组LEA蛋白可以赋予对冰冻胁迫的抗性。讨论了观察到的非生物胁迫抗性改善的可能机制。 (摘要由UMI缩短。)

著录项

  • 作者

    Yongil, Yang.;

  • 作者单位

    University of Nevada, Reno.;

  • 授予单位 University of Nevada, Reno.;
  • 学科 Biology Plant Physiology.
  • 学位 M.S.
  • 年度 2004
  • 页码 149 p.
  • 总页数 149
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

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