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首页> 外文学位 >Simultaneous determination of nitrate and nitrite with a paper-based microfluidic device.
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Simultaneous determination of nitrate and nitrite with a paper-based microfluidic device.

机译：用纸基微流控设备同时测定硝酸盐和亚硝酸盐。

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摘要

Microfluidic devices for detecting nitrate and nitrite ion in water samples were developed. Nitrate and nitrite were chosen for their environmental impact, as established in other research. High levels of nitrate and nitrite ions are known to cause eutrophication in small water bodies. In human beings they can increase the occurrence of methemoglobinemia. Paper was chosen as the substrate for preparing these devices as it is easy to handle and cost effective. Hydrophobic (wax) barriers printed on paper define channels through which samples can flow, mixing with reagents.;Nitrite was detected by diazotization in the Griess assay reaction, which gave a pink color. Nitrate was detected by the same reaction after it was reduced to nitrite ion by vanadium (III) chloride within the sensor device. Quantitative measurements were possible based on the intensity of the color formed. The devices were scanned after drying to obtain the color values. The RGB color model was chosen to represent the color reflected from the devices. It was observed that the concentration of nitrate and nitrite was inversely proportional to the green color component (measuring the pink absorbance lambdamax = 540nm). Reflectance spectroscopy confirmed the inverse correlation.;A calibration was developed using nitrite standards and the amount of nitrite and nitrate were determined in drinking water and soil samples. The sensors were able to detect 15-ppm nitrate and 5-ppm nitrite in drinking water samples.
机译：开发了用于检测水样中硝酸根和亚硝酸根离子的微流体装置。如其他研究所确定的，选择硝酸盐和亚硝酸盐是因为它们对环境具有影响。众所周知，硝酸盐和亚硝酸盐离子含量高会导致小型水体富营养化。在人类中，它们可以增加高铁血红蛋白血症的发生。选择纸作为制备这些装置的基材，因为它易于处理且具有成本效益。印刷在纸张上的疏水性（蜡质）阻隔层限定了样品流过的通道，可与试剂混合。亚硝酸盐在Griess分析反应中通过重氮化进行检测，呈粉红色。硝酸盐在传感器装置内被氯化钒（III）还原为亚硝酸根离子后，通过相同的反应进行检测。可以根据形成的颜色的强度进行定量测量。干燥后扫描设备以获得颜色值。选择RGB颜色模型来代表从设备反射的颜色。观察到硝酸盐和亚硝酸盐的浓度与绿色分量成反比（测量粉红色吸光度λmax= 540nm）。反射光谱法证实了这种反相关。使用亚硝酸盐标准品进行校准，并测定饮用水和土壤样品中的亚硝酸盐和硝酸盐含量。这些传感器能够检测饮用水样品中的15 ppm硝酸盐和5 ppm亚硝酸盐。

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  • 作者

    Ummadi, Jyothir Ganeshwar Reddy.;

  • 作者单位

    Tennessee Technological University.;

  • 授予单位 Tennessee Technological University.;
  • 学科 Chemistry.;Inorganic chemistry.;Analytical chemistry.
  • 学位 M.S.
  • 年度 2012
  • 页码 85 p.
  • 总页数 85
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 地下建筑;
  • 关键词

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