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首页> 外文学位 >Exploratory Analysis of Pathogen Resistance Responsible Genetic Elements in Two Eudicots from Whole Genome Sequence.
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Exploratory Analysis of Pathogen Resistance Responsible Genetic Elements in Two Eudicots from Whole Genome Sequence.

机译：对全基因组序列中两个双子叶植物病原菌抗性负责的遗传因素的探索性分析。

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摘要

Agricultural products are becoming more homogenous in order to conform to consumer expectations. In doing so, they have become a high-risk target for widespread crop failure due to pathogens or other agricultural maladies. Presented here are a collection of tools and a work-flow for the wholesale detection and characterization of disease resistance-associated proteins from whole-genome sequences. These techniques were also adapted for identifying potential miRNA regulatory sequences.;Putative R-gene sequences identified from the Malus x Domestica 'Golden Delicious' genome were acquired for verification against wild apple species. Resistance gene analogs were PCR amplified from R-gene associated domains (TIR, NBS, and LRR) in wild apple cultivars. Known R-gene sequences were clustered alongside the PCR analogs and putative R-genes from 'Golden Delicious'.;A covariance model approach for the de novo detection of 3,187 putative pre-miRNA regulatory sequences is also explored. Vitis vinifera sequences used to build this model were retrieved with 38% efficiency.;Computational clusters may be useful in the physical mapping of sequences to the chromosomally duplicated gene clusters characteristic of R-genes. Known R-genes included in computational clusters may also clarify the function of the unknown sequences. A possibility exists for the identification of resistance genes that have been lost in the selective breeding of commercial cultivars.
机译：为了符合消费者的期望，农产品变得越来越同质。这样做，它们已成为由于病原体或其他农业疾病而导致广泛的农作物歉收的高风险目标。这里介绍了用于从全基因组序列中全面检测和鉴定与疾病抗性相关的蛋白质的工具和工作流程的集合。这些技术还适用于鉴定潜在的miRNA调控序列。获得了从苹果属（Malus）x Domestica'金冠'（Golden Delicious）基因组中鉴定的R基因序列，以针对野生苹果物种进行验证。从野生苹果品种的R基因相关域（TIR，NBS和LRR）PCR扩增抗性基因类似物。已知的R基因序列与'Golden Delicious'的PCR类似物和推定的R基因一起聚类。还探索了从头检测3,187个推定的pre-miRNA调控序列的协方差模型方法。以38％的效率检索了用于构建该模型的葡萄序列。;计算簇可能对序列到R基因特征的染色体复制基因簇的物理作图很有用。计算簇中包含的已知R基因也可以阐明未知序列的功能。存在鉴定在商业品种的选择性育种中丢失的抗性基因的可能性。

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  • 作者

    Bailey, Aubrey.;

  • 作者单位

    Rochester Institute of Technology.;

  • 授予单位 Rochester Institute of Technology.;
  • 学科 Biology Molecular.;Agriculture Plant Pathology.;Biology Bioinformatics.
  • 学位 M.S.
  • 年度 2011
  • 页码 55 p.
  • 总页数 55
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 公共建筑;
  • 关键词

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