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A quantitative, technology independent, fidelity metric for evaluating bioprinted patterned co-cultures.

机译：一种定量的，与技术无关的保真度指标，用于评估生物印刷的图案共培养。

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摘要

The study of cell-cell interactions is crucial in the understanding of cell behaviors such as tumor genesis, proliferation, migration, metastasis, and apoptosis. To break down the complex web of signals in vivo, researchers must replicate some parts of this environment with in vitro tissue test systems, composed of multiple cell types arranged close enough to communicate with their neighbors, i.e. high-resolution co-culture patterns. The field of bioprinting is specifically focused on creating co-culture patterns for the purposes of cell studies, but the sample resolutions of most bioprinting systems are still too coarse to permit cell communication. No way currently exists to compare the sample fidelity between the technologies that have succeeded in creating high-resolution co-culture patterns.;This work introduces a quantitative metric for measuring co-culture patterning fidelity for use in comparing systems or tracking changes in fidelity with experiment conditions. The "biopatterning fidelity index" (BFI) measures the performance of a system by fitting a scaled mask of the sample pattern over an image of the printed pattern and classifying the cells as correctly or incorrectly placed. A simple model is also introduced to provide a theoretical upper bound on the expected fidelity. The BFI and model were used to assess the performance of a custom bioprinter system. The performance of the system varied between the different cell types. The results indicate that the post-processing procedures were disturbing the fidelity of the patterns. New procedures should be developed that would not disturb the initial pattern fidelity. The best samples came very close to the model's predicted upper bound. As the number of capable technologies increases, the BFI will provide a quantitative, technology-independent method to assess the fidelity of patterned co-cultures.;The last section of this work examines the ability of the bioprinting system to create multiple slides of samples with similar cell distributions. It was shown that cartridges which had been exposed to less usage and cleaning had a more consistent cell output, enabling the bioprinting system to create biological comparable samples.
机译：细胞间相互作用的研究对于理解诸如肿瘤发生，增殖，迁移，转移和凋亡的细胞行为至关重要。为了在体内分解复杂的信号网，研究人员必须使用体外组织测试系统复制该环境的某些部分，该系统由多种细胞类型组成，这些细胞类型的排列应足够近以与邻居沟通，即高分辨率共培养模式。生物打印领域特别专注于创建用于细胞研究目的的共培养模式，但是大多数生物打印系统的样品分辨率仍然太粗糙，无法进行细胞通讯。当前尚无方法可以比较成功创建高分辨率共培养模式的技术之间的样本保真度。这项工作引入了一种定量度量，用于测量共培养模式保真度，用于比较系统或跟踪保真度变化。实验条件。 “生物图案保真度指数”（BFI）通过在打印图案的图像上安装样品图案的缩放蒙版并将细胞正确或错误地分类，来测量系统的性能。还引入了一个简单的模型来提供预期保真度的理论上限。 BFI和模型用于评估定制生物打印机系统的性能。系统的性能在不同类型的电池之间变化。结果表明，后处理程序干扰了图案的保真度。应该开发不会干扰初始模式保真度的新程序。最佳样本非常接近模型的预测上限。随着功能强大的技术数量的增加，BFI将提供一种与技术无关的定量方法，以评估带图案的共培养物的保真度。这项工作的最后一部分探讨了生物打印系统创建带有多个样本的载玻片的能力。类似的细胞分布。结果表明，较少使用和清洁的墨盒具有更稳定的细胞输出，从而使生物打印系统能够创建生物学可比的样品。

著录项

  • 作者

    Pepper, Matthew E.;

  • 作者单位

    Clemson University.;

  • 授予单位 Clemson University.;
  • 学科 Engineering Biomedical.;Engineering Electronics and Electrical.
  • 学位 M.S.
  • 年度 2011
  • 页码 122 p.
  • 总页数 122
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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