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首页> 外文学位 >Evaluation of reduced toxicity of deoxynivalenol and zearalenone by extrusion processing as determined by the MTT bioassay using mammalian cell cultures.
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Evaluation of reduced toxicity of deoxynivalenol and zearalenone by extrusion processing as determined by the MTT bioassay using mammalian cell cultures.

机译：通过挤压加工评估脱氧雪腐烯醇和玉米赤霉烯酮的毒性降低，如通过MTT生物测定法使用哺乳动物细胞培养物测定的。

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摘要

The most sensitive mammalian cell lines to Fusarium mycotoxins, deoxynivalenol (DON), zearalenone (ZEN), fumonisin (FUM), and moniliformin (MON), were investigated for further toxicological investigations as alternatives to whole animal testing for screening extracts of Fusarium spp. contaminated foods and feeds. The Chinese hamster ovary (CHO-K1) for DON and FUM, hepatocel lular carcinoma (HepG2) for MON, and Balb/c mice keratinocyte (C5-O) for ZEN were found to be the most sensitive cell lines with IC50 values of 0.27, 85.5, 26.8, and 24.1 mug/ml, respectively, as measured by the MTT bioassay. In the following study, reduced DON concentrations in contaminated corn grits after extrusion were analyzed by chemical (HPLC) and biochemical (ELISA) methods. The loss of toxicity of DON was confirmed by the MTT bioassay using the CHO-K1 cell line. Extrusion temperatures of 160, 180, 200 and 220°C and screw speeds of 50, 80, 110 and 140 rpm significantly reduced DON levels in contaminated corn grits by 22--35% (P 0.05). A second extrusion processing was designed to evaluate reduction of levels of both DON and ZEN in F. graminearum contaminated corn grits. This was analyzed by HPLC and ELISA, and reduced toxicity a was confirmed by the MTT bioassay. The CHO-K1 cells was used to evaluate the cytotoxicity of DON, whereas the adenocarcinoma human breast cells (MCF-7), which has estrogen receptors, was used to evaluate the estrogenic activity of ZEN. The effect of extrusion temperatures of 150, 175, and 200°C was found to be a linear, with DON reduced by 22--35%, whereas a quadratic response on reduced ZEN by 67--81%. The MTT bioassay results were more closely correlated with the HPLC results (r = 0.90 for DON, r = 0.96 for ZEN) than the ELISA results (r = 0.78 for DON, 0.83 for ZEN). The MTT bioassay was proved to be a useful method for quantification of DON and ZEN, as well as a potential toxicity screening method for contaminated extruded cereal based products.
机译：研究了对镰刀菌霉菌毒素，脱氧雪腐烯醇（DON），玉米赤霉烯酮（ZEN），伏马菌素（FUM）和莫尼西菌素（MON）最敏感的哺乳动物细胞系，以作进一步的毒理学研究，以替代全动物试验以筛选镰刀菌属提取物。被污染的食物和饲料。发现DON和FUM的中国仓鼠卵巢（CHO-K1），MON的肝细胞肝癌（HepG2）和ZEN的Balb / c小鼠角质形成细胞（C5-O）是最敏感的细胞系，IC50值为0.27通过MTT生物测定法分别测得分别为85.5、26.8和24.1杯/毫升。在以下研究中，通过化学（HPLC）和生化（ELISA）方法分析了挤压后受污染的玉米粉中降低的DON浓度。使用CHO-K1细胞系通过MTT生物测定法证实了DON的毒性丧失。 160、180、200和220°C的挤出温度以及50、80、110和140 rpm的螺杆转速可将受污染的玉米粉中的DON水平显着降低22--35％（P <0.05）。设计了第二个挤压工艺，以评估受禾谷镰刀菌污染的玉米粗粉中DON和ZEN含量的降低。通过HPLC和ELISA对其进行分析，并且通过MTT生物测定法确认毒性降低。 CHO-K1细胞用于评估DON的细胞毒性，而具有雌激素受体的腺癌人乳腺癌细胞（MCF-7）用于评估ZEN的雌激素活性。发现挤出温度在150、175和200°C时呈线性关系，DON降低22--35％，而ZEN降低二次响应67-81％。 MTT生物测定结果与HPLC结果（DON的r = 0.90，ZEN的r = 0.96）与ELISA结果的结果（DON的r = 0.78，ZEN的0.83）更密切相关。事实证明，MTT生物测定法是定量DON和ZEN的有用方法，也是对受污染的谷物挤压产品的潜在毒性筛选方法。

著录项

  • 作者

    Cetin, Yuksel.;

  • 作者单位

    The University of Nebraska - Lincoln.;

  • 授予单位 The University of Nebraska - Lincoln.;
  • 学科 Agriculture Food Science and Technology.; Health Sciences Toxicology.
  • 学位 Ph.D.
  • 年度 2003
  • 页码 228 p.
  • 总页数 228
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农产品收获、加工及贮藏;毒物学（毒理学）;
  • 关键词

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