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首页> 外文期刊>Journal of Agricultural and Food Chemistry >Confirmation of reduced toxicity of deoxynivalenol in extrusion-processed corn grits by the MTT bioassay
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Confirmation of reduced toxicity of deoxynivalenol in extrusion-processed corn grits by the MTT bioassay

机译：通过MTT生物测定法确认脱氧雪腐烯醇在挤压加工的玉米粗粉中的毒性降低

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摘要

The objective of this study was to determine the loss of toxicity of deoxynivalenol in extruded cereal-based products by the tetrazolium salt (MTT) bioassay using a sensitive Chinese hamster ovary (CHO-K1) cell line and to compare the results to chemical (high-performance liquid chromatography, HPLC) and biochemical (enzyme-linked immunosorbant assay, ELISA) methods of analysis. A split-split plot design was used for the extrusion process experiments at temperatures of 150, 175, and 200 degrees C and screw speeds of 70 and 140 rpm. The initial mean deoxynivalenol concentration in the corn grits artificially contaminated with Fusarium graminearum was found to be 23.5 mug/g as measured by HPLC. The percent reductions of deoxynivalenol in the contaminated corn grits upon extrusion processing ranged from 22 to 35%, from 21 to 34%, and from 21 to 37% as measured by HPLC, ELISA, and MTT bioassay, respectively. The MTT bioassay results were more closely correlated with HPLC (r = 0.90) results than with ELISA results (r = 0.78). The MTT bioassay, using a sensitive mammalian cell line, was demonstrated to be a useful method for quantification of deoxynivalenol as well as a potential toxicity screening method for contaminated extruded cereal-based products.
机译：这项研究的目的是使用敏感的中国仓鼠卵巢（CHO-K1）细胞系，通过四唑盐（MTT）生物测定法确定谷物基产品中脱氧雪腐烯醇的毒性损失，并将结果与​​化学法（高高效液相色谱，HPLC）和生化（酶联免疫吸附测定，ELISA）分析方法。在150、175和200摄氏度的温度下以及70和140 rpm的螺杆速度下，将分流图设计用于挤出过程实验。经HPLC测定，发现被禾谷镰刀菌人工污染的玉米粗粉中的初始平均脱氧雪腐烯醇浓度为23.5杯/克。通过HPLC，ELISA和MTT生物测定法测得，挤压加工后受污染的玉米粉中脱氧雪腐烯醇的减少百分比分别为22％至35％，21％至34％和21％至37％。 MTT生物测定结果与HPLC结果（r = 0.78）的相关性与HPLC（r = 0.90）密切相关。使用敏感的哺乳动物细胞系进行的MTT生物测定已被证明是定量脱氧雪腐烯醇的有用方法，也是对受污染的谷物挤压产品的潜在毒性筛选方法。

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