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首页> 外文学位 >The effects of 24R, 25-dihydroxyvitamin D3 and 24S, 25- dihydroxyvitamin D3 on phosphate transport in vivo.
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The effects of 24R, 25-dihydroxyvitamin D3 and 24S, 25- dihydroxyvitamin D3 on phosphate transport in vivo.

机译：24R，25-二羟基维生素D3和24S，25-二羟基维生素D3对体内磷酸盐转运的影响。

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摘要

The steroid hormone 1,25-dihydroxyvitamin D3 [1,25(OH) 2D3] rapidly stimulates the uptake of phosphate in isolated chick intestinal cells, while the steroid 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] inhibits the rapid stimulation by 1,25(OH)2D 3. Previous work in this laboratory has indicated that 24,25(OH) 2D3 inhibits phosphate uptake in isolated intestinal cells and perfused duodenal loops. It is critical to show this effect in the whole animal to determine the presence of any confounding factors. Studies were therefore undertaken to determine if 24,25(OH)2D3 had a similar effect in vivo. 24,25(OH)2D3 has two isomers which are 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D 3] and 24S,25-dihydroxyvitamin D3 [24S,25(OH)2D 3]. We studied these two isomers separately and tested them over a time course of 1, 5, 10, 15 and 18 hours after steroid using chicks on regular diet, but fasted, and chicks on a lower vitamin D diet. All chicks were anesthetized prior to surgical exposure of the duodenal loop and injection of a solution containing H332PO4 into the lumen. An initial time course study of phosphate transport determined that 3 to 9 min of absorption in vivo was in a linear range, as judged by serum levels of radioactivity. Chicks were then injected with either 200 microg 24R,25(OH) 2D3, 20 microg 24S,25(OH)2D3 or vehicle for control groups within the same time course studies. We found that the isomers had different effects on phosphate absorption. 24R,25(OH)2D 3 had a hypophosphatemic effect in vivo. The serum levels of radionuclide revealed hypophosphatemic effects at 1, 5, 15 and 18 hours time points with a decrease of 56%, 42%, 39% and 43%, respectively, (P 0.05) compared with controls. In contrast, 24S,25(OH)2D3 stimulated intestinal phosphate absorption at the five hour time point by 64%, but had no other effects at the other time points tested.
机译：类固醇激素1,25-二羟基维生素D3 [1,25（OH）2D3]迅速刺激分离的鸡肠道细胞中磷酸盐的摄取，而类固醇24,25-二羟基维生素D3 [24,25（OH）2D3]抑制1,25（OH）2D 3快速刺激。3.该实验室先前的研究表明，24,25（OH）2D3抑制离体肠细胞和灌注的十二指肠环中的磷酸盐吸收。至关重要的是要在整个动物中显示这种效应，以确定是否存在任何混杂因素。因此，进行了研究以确定24,25（OH）2D3是否在体内具有相似的作用。 24,25（OH）2D3具有两个异构体，分别是24R，25-二羟基维生素D3 [24R，25（OH）2D 3]和24S，25-二羟基维生素D3 [24S，25（OH）2D 3]。我们分别研究了这两种异构体，并在类固醇注射后的1、5、10、15和18小时的时间范围内对它们进行了测试，使用的是常规饮食但禁食的雏鸡和低维生素D饮食的雏鸡。在手术暴露十二指肠环并将内含H332PO4的溶液注入内腔之前，先麻醉所有小鸡。根据血清放射性水平判断，磷酸盐转运的最初时间过程研究确定体内3至9分钟的吸收处于线性范围。然后在同一时程研究中，给小鸡注射200 microg 24R，25（OH）2D3、20 microg 24S，25（OH）2D3或媒介物。我们发现异构体对磷酸盐的吸收有不同的影响。 24R，25（OH）2D 3具有体内降血磷作用。与对照组相比，放射性核素的血清水平在1、5、15和18小时的时间点显示出低磷酸盐作用，分别降低了56％，42％，39％和43％（P <0.05）。相反，24S，25（OH）2D3在五个小时的时间点刺激了肠道磷酸盐的吸收，增加了64％，但在其他测试的时间点没有其他影响。

著录项

  • 作者

    Meng, Yu.;

  • 作者单位

    Utah State University.;

  • 授予单位 Utah State University.;
  • 学科 Agriculture General.;Health Sciences Nutrition.
  • 学位 M.S.
  • 年度 2011
  • 页码 48 p.
  • 总页数 48
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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