• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Zein protein interactions and stabilization: Utilizing high-methionine zein proteins to improve the nutritional quality of vegetative plant tissues.
【24h】

Zein protein interactions and stabilization: Utilizing high-methionine zein proteins to improve the nutritional quality of vegetative plant tissues.

机译：玉米醇溶蛋白相互作用和稳定化：利用高甲硫氨酸玉米醇溶蛋白来改善植物营养植物的营养质量。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Expression of the 18-kD zein gene under the control of the CaMV 35S promoter results in stable transcript and protein accumulation in leaf tissues of transgenic tobacco. Co-expression of the 15-kD and 18-kD zein genes followed by immunolocalization revealed that both proteins accumulate in the same protein bodies. Furthermore, there is a 16-fold increase in accumulation of the 18-kD zein protein in co-expressing tobacco plants. Transgenic tobacco leaves co-expressing the 15-kD and 18-kD zein genes, or expressing only the 18-kD zein gene, accumulate the 18-kD zein transcript to equal levels. This suggests that the 15-kD zein protein stabilizes the 18-kD zein protein when sequestered in protein bodies. The possibility of 18-kD zein protein stabilization by the 15-kD zein protein is further evidenced by L-[35S] methionine pulse-chase experiments. A dramatic decrease in the rate of 18-kD protein degradation in transgenic tobacco leaves co-expressing the 15-kD and 18-kD zein genes was observed when compared to 18-kD protein degradation in transgenic tobacco leaves expressing only the 18-kD zein gene.; Promoter analysis studies were peformed on a chimeric, bi-directional mannopine synthase (mas) promoter with an enhancer element from the octopine synthase (ocs) gene, called ΔMAS-IOCS. The ΔMAS-1OCS promoter was used to control expression of the 15-kD β-zein gene in transgenic tobacco leaf tissues. Regulation of β-zein expression was observed in response to endogenous and environmental factors in the transgenic tobacco leaf tissues, with highest expression occurring in leaf tissues nearest the plant base and in roots. Analysis of the promoter sequence revealed an AS-1 motif and a root-specific expression motif of the rolD promoter of A. rhizogenes.; Amino acid analysis of T2 transgenic tobacco leaf tissues co-expressing the 15-kD and 18-kD zein genes showed an average increase in bound methionine of ∼11% when compared with non-transformed tobacco leaf tissues, and accumulated zein proteins to ∼4.1% of total leaf protein. Accumulating the 15-kD and 18-kD zein proteins to 4.1% of total alfalfa leaf protein should increase the methionine content such that alfalfa forage alone would supply adequate levels of all 10 essential amino acids required for cattle growth and milk production.
机译：在CaMV 35S启动子控制下18-kD玉米醇溶蛋白基因的表达导致转基因烟草叶片组织中稳定的转录本和蛋白质积累。 15-kD和18-kD玉米醇溶蛋白基因的共表达，然后进行免疫定位，发现这两种蛋白都积累在相同的蛋白体中。此外，在共表达烟草植物中18 kD玉米醇溶蛋白的积累增加了16倍。共表达15-kD和18-kD玉米醇溶蛋白基因或仅表达18-kD玉米醇溶蛋白基因的转基因烟叶将18-kD玉米醇溶蛋白转录本积累到相同水平。这表明15-kD玉米醇溶蛋白被螯合在蛋白质体内时可以稳定18-kD玉米醇溶蛋白。 L-[ 35 S]蛋氨酸脉冲追踪实验进一步证明了15-kD玉米醇溶蛋白稳定18-kD玉米醇溶蛋白的可能性。与仅表达18-kD玉米醇溶蛋白的转基因烟草叶片中的18-kD蛋白降解相比，共表达15-kD和18-kD玉米醇溶蛋白基因的转基因烟草叶片中18-kD蛋白降解速率显着降低。基因。;启动子分析研究是在嵌合，双向甘露聚糖合酶（ mas ）启动子上进行的，该启动子具有来自章鱼碱合酶（ ocs ）基因的增强子，称为ΔMAS-IOCS。 ΔMAS-1OCS启动子用于控制15-kDβ-玉米醇溶蛋白基因在转基因烟草叶片组织中的表达。在转基因烟草叶组织中，观察到β-玉米醇溶蛋白表达的调节是响应于内源性和环境因素的，其中最高的表达发生在距植物基部最近的叶组织和根中。启动子序列分析表明，发根农杆菌的rolD启动子具有AS-1基序和根特异性表达基序。共表达15-kD和18-kD玉米醇溶蛋白基因的T2转基因烟叶组织的氨基酸分析显示，与未转化的烟叶组织相比，结合的蛋氨酸平均增加了约11％，玉米醇溶蛋白累积到约4.1占叶片总蛋白的百分比。将15-kD和18-kD玉米醇溶蛋白积累到苜蓿叶蛋白总量的4.1％，应增加蛋氨酸含量，这样仅苜蓿草料就能提供足够水平的牛生长和产奶所需的全部10种必需氨基酸。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号