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首页> 外文学位 >Cell wall degrading enzymes and interaction between Trichoderma aggressivum and Agaricus bisporus.
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Cell wall degrading enzymes and interaction between Trichoderma aggressivum and Agaricus bisporus.

机译：细胞壁降解酶及其在木霉和双孢蘑菇之间的相互作用。

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摘要

Agaricus bisporus is the most commonly cultivated mushroom in North America and has a great economic value. Green mould is a serious disease of A. bisporus and causes major reductions in mushroom crop production. The causative agent of green mould disease in North America was identified as Trichoderma aggressivum f. aggressivum. Variations in the disease resistance have been shown in the different commercial mushroom strains.;First, we tried to isolate and sequence the N-acetylglucosaminidase from A. bisporus to understand the defensive mechanism of mushroom against the disease. However, the lack of genomic and proteomic information of A. bisporus limited our efforts. Next, T. aggressivum cell wall degrading enzymes that are thought to attack Agaricus and mediate the disease development were examined. The three cell wall degrading enzymes genes, encoding endochitinase (ech42), glucanase (beta-1,3 glucanase) and protease (prb1), were isolated and sequenced from T. aggressivum f. aggressivum. The sequence data showed significant homology with the corresponding genes from other fungi including Trichoderma species. The transcription levels of the three T. aggressivum cell wall degrading enzymes were studied during the in vitro co-cultivation with A. bisporus using RT-qPCR. The transcription levels of the three genes were significantly upregulated compared to the solitary culture levels but were upregulated to a lesser extent in co-cultivation with a resistant strain ofA. bisporus than with a sensitive strain.;An Agrobacterium tumefaciens transformation system was developed for T. aggressivum and was used to transform three silencing plasmids to construct three new T. aggressivum phenotypes, each with a silenced cell wall degrading enzyme. The silencing efficiency was determined by RT-qPCR during the individual in vitro co-cultivation of each of the new phenotypes with A. bisporus . The results showed that the expression of the three enzymes was significantly decreased during the in vitro co-cultivation when compared with the wild type. The phenotypes were co-cultivated with A. bisporus on compost with monitoring the green mould disease progression. The data indicated that prb1 and ech42 genes is more important in disease progression than the beta-1,3 glucanase gene. Finally, the present study emphasises the role of the three cell wall degrading enzymes in green mould disease infection and may provide a promising tool for disease management.;The purpose of this study is to continue investigations of the interactions between T. aggressivum and A. bisporus during the development of green mould disease. The main focus of the research was to study the roles of cell wall degrading enzymes in green mould disease resistance and pathogenesis.
机译：双孢蘑菇是北美最常见的蘑菇，具有很高的经济价值。绿色霉菌是双孢曲霉的一种严重疾病，会导致蘑菇作物产量的大幅下降。在北美，导致绿色霉菌病的病原被鉴定为a。Trichoderma aggressivum f。侵略性的在不同的商业蘑菇菌株中已显示出抗病性的变化。首先，我们试图从双孢曲霉中分离和测序N-乙酰氨基葡糖苷酶，以了解蘑菇对这种疾病的防御机制。但是，缺乏双孢蘑菇的基因组和蛋白质组学信息限制了我们的努力。接下来，研究了被认为攻击姬松茸并介导疾病发展的侵略性T. agressiveum细胞壁降解酶。从a.gression afgressive f。分离并测序了三种细胞壁降解酶基因，分别编码内切壳聚糖酶（ech42），葡聚糖酶（β-1,3葡聚糖酶）和蛋白酶（prb1）。侵略性的序列数据显示与来自其他真菌（包括木霉属）的相应基因具有显着同源性。使用RT-qPCR在与双孢曲霉体外共培养过程中研究了三种侵略性锥虫细胞壁降解酶的转录水平。与单独的培养水平相比，这三个基因的转录水平显着上调，但在与A耐药菌株的共培养中被上调的程度较小。开发了一种农杆菌转化农杆菌的系统，该农杆菌转化系统用于转化农杆菌，用于转化三个沉默质粒，以构建三种新的农杆菌表型，每个表型均具有沉默的细胞壁降解酶。在每种新表型与双孢曲霉的体外共培养过程中，通过RT-qPCR测定沉默效率。结果表明，与野生型相比，三种酶的表达在体外共培养过程中明显降低。该表型与双孢蘑菇在堆肥上共培养，并监测绿色霉菌病的进展。数据表明prb1和ech42基因在疾病进展中比β-1,3葡聚糖酶基因更重要。最后，本研究强调了三种细胞壁降解酶在绿色霉菌疾病感染中的作用，并可能为疾病管理提供有前途的工具。;本研究的目的是继续研究侵略性T.aggressivum和A.之间的相互作用。双孢霉菌在绿色霉菌病发展过程中。研究的主要焦点是研究细胞壁降解酶在绿色霉菌抗病性和发病机理中的作用。

著录项

  • 作者

    Abubaker, Kamal Salem.;

  • 作者单位

    Brock University (Canada).;

  • 授予单位 Brock University (Canada).;
  • 学科 Biology Microbiology.;Agriculture Plant Pathology.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 193 p.
  • 总页数 193
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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