• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Chiral studies of non-covalent complexes by electrospray ionization (ESI) Fourier transform mass spectrometry (FTMS).
【24h】

Chiral studies of non-covalent complexes by electrospray ionization (ESI) Fourier transform mass spectrometry (FTMS).

机译：通过电喷雾电离（ESI）傅里叶变换质谱（FTMS）对非共价配合物进行手性研究。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The introductory chapter touches upon the significance chirality carries in life, presenting cyclodextrin and maltoheptaose as hosts for the enantiomeric studies carried out throughout this work.; The chirality studies are based solely on mass spectrometry, employing a gas-phase exchange reaction between a non-covalent complex, [Host:Guest+H] +, and a base. The theory and instrumentation of electrospray ionization (ESI) source and Fourier transform mass spectrometry (FTMS) are also included.; The second chapter introduces the method of Peak Height Analysis, for determining enantiomeric excess in mixtures of amino acids, based solely mass spectrometry. It is based on a gas-phase ion/molecule reaction, between a protonated complex and an alkyl amine is used. The complex is comprised of a β-cyclodextrin host and an amino acid guest that is replaced by the alkyl amine. The rates of the guest exchange reaction vary with the chirality of the amino acid. A calibration curve is produced and the fractions of the D isomer in the test mixtures are determined.; Chapter three presents practical applications of the Peak Height Analysis method in the previous chapter. Protonated complexes involving cyclodextrin hosts and pharmaceuticals as the guests are used. The pharmaceutical compounds include DOPA, amphetamine, ephedrine, and penicillamine. Enantiomeric excess was determined via this method. To understand the nature of the interactions, molecular dynamics calculations are used.; In chapter four, the enantiomeric excess determination method is extended to an ion trap mass spectrometer. IITMS is employed to analyze chiral guest-exchange reactions in gas-phase complexes of amino acids and cyclodextrin. Enantioselectivity was obtained with several amino acids. The results are consistent with those obtained earlier with Fourier transform mass spectrometry (FTMS). The two mass analyzers are compared with regard to enantiomeric analysis.; Chapter five takes a more theoretical approach, using the enantioselective exchange reaction to study and understand the enantiomeric complex behavior in the gas phase using only mass spectrometry. Protonated complexes comprised of guest peptides with permethylated hosts cyclodextrin and maltoheptaose are produced in the gas phase and reacted with a gaseous alkyl amine. Influence of both host and guest size, of alkyl amine gas-phase basicity, and other factors are investigated. Molecular dynamics calculations are used to aid in elucidating reactivities of the exchange reactions.
机译：介绍性章节涉及手性在生活中的意义，介绍了环糊精和麦芽七糖作为整个工作中对映体研究的宿主。手性研究仅基于质谱法，利用非共价复合物[Host：Guest + H] + 与碱之间的气相交换反应。还包括电喷雾电离（ESI）源和傅里叶变换质谱（FTMS）的理论和仪器。第二章介绍了仅基于质谱法测定氨基酸混合物中对映体过量的峰高分析方法。它基于气相离子/分子反应，在质子化的配合物和烷基胺之间使用。该复合物由β-环糊精主体和被烷基胺取代的氨基酸客体组成。客体交换反应的速率随氨基酸的手性而变化。产生标准曲线，并确定测试混合物中D异构体的分数。第三章介绍了上一章中“峰高分析”方法的实际应用。使用客体时涉及环糊精主体和药物的质子化复合物。药物化合物包括DOPA，苯丙胺，麻黄碱和青霉素。通过该方法确定对映体过量。为了理解相互作用的性质，使用了分子动力学计算。在第四章中，将对映体过量测定方法扩展到离子阱质谱仪。 IITMS用于分析氨基酸和环糊精的气相复合物中的手性客体交换反应。用几种氨基酸获得对映选择性。结果与先前使用傅里叶变换质谱（FTMS）获得的结果一致。比较两个质量分析仪的对映体分析。第五章采用更理论的方法，仅通过质谱法使用对映体选择性交换反应来研究和理解气相中的对映体复杂行为。在气相中产生由客体肽与全甲基化的主体环糊精和麦芽七糖组成的质子化复合物，并使其与气态烷基胺反应。研究了主体和客体尺寸，烷基胺气相碱度的影响以及其他因素。分子动力学计算用于帮助阐明交换反应的反应性。

著录项

  • 作者

    Grigorean, Gabriela.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 141 p.
  • 总页数 141
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号