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首页> 外文学位 >Epidemiological typing of Campylobacter clinical and food isolates using pulsed-field gel electrophoresis.
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Epidemiological typing of Campylobacter clinical and food isolates using pulsed-field gel electrophoresis.

机译：使用脉冲场凝胶电泳对弯曲杆菌临床和食品分离株进行流行病学分型。

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摘要

Campylobacteriosis is the most frequently reported type of acute bacterial gastroenteritis in Canada. In 2000 alone, 11,846 campylobacteriosis cases were reported in Canada. The majority of these cases are sporadic, and their causes remain unknown. An attempt was made to gain a better understanding of the epidemiology of campylobacteriosis, both by identifying foods and environments that harbor Campylobacter spp., and by characterizing clinical, food and environmental isolates using pulsed-field gel electrophoresis (PFGE). Spot maps were also used to determine the geographical relationship of these campylobacteriosis cases.; A variety of raw and ready-to-eat foods were tested for the presence of Campylobacter spp. From the 300 samples analyzed, Campylobacter spp. were detected in four samples, one raw beef liver sample and three raw chicken samples. An isolation rate of 9.7% was observed among the raw chicken samples tested, a significantly-reduced percentage, as compared to a 1981 Canadian survey. The prevalence of Campylobacter spp. in a poultry foodservice operation in Ottawa, was also determined from March to August 2001. No Campylobacter spp. were detected in the 125 samples tested.; Campylobacter clinical and food isolates were characterized using PFGE with two restriction enzymes, SmaI and KpnI. KpnI produced more complex banding patterns than SmaI, and proved to be more discriminatory. Among the 154 isolates assigned to clusters by SmaI, only 42% gave concordant results with KpnI. In contrast, among the 53 isolates assigned to 23 clusters by KpnI, 87% gave concordant results with SmaI. Five of the 20 concordant clusters represented isolates obtained from the same person, suggesting that some of these individuals may have become re-infected.; Spot map analysis revealed a significant clustering of campylobacteriosis cases in the former city of Ottawa, most of which, did not belong to the same postal code. In contrast, very few cases were observed in outlying regions; however, most of these cases belonged to the same postal code, suggesting the possible presence of local outbreaks.
机译：弯曲杆菌病是加拿大最常报告的急性细菌性胃肠炎。仅在2000年，加拿大报告了11846例弯曲杆菌病病例。这些病例大多数是零星的，其原因仍然未知。试图通过识别带有 Campylobacter spp。的食物和环境，以及使用脉冲场凝胶电泳对临床，食物和环境分离物进行鉴定，从而更好地了解弯曲菌的流行病学（ PFGE）。斑点地图还被用来确定这些弯曲菌病病例的地理关系。测试了各种生食和即食食品中是否存在 Campylobacter spp。从分析的300个样本中，弯曲杆菌 spp。在四份样本中检出，其中一份为生牛肉肝样本，三份为鸡肉样本。与1981年加拿大调查相比，在所测试的生鸡肉样本中，检出率为9.7％，大大降低了百分比。 Campylobacter spp的患病率。还确定了从2001年3月至8月在渥太华的一家禽类食品服务运营中进行的操作。没有 Campylobacter spp。在125个测试样品中被检测到。使用带有两种限制性酶 Sma I和 Kpn I的PFGE对弯曲杆菌的临床和食品分离株进行了表征。与 Sma I相比， Kpn I产生了更复杂的条带模式，并被证明具有更大的歧视性。在 Sma I分配给簇的154个分离物中，只有42％的结果与 Kpn I一致。相反，在由 Kpn I分配给23个簇的53个分离株中，有87％的结果与 Sma I一致。 20个一致的簇中有5个代表从同一个人获得的分离株，这表明其中一些个体可能已被再次感染。现场地图分析显示，渥太华前城市存在大量的弯曲杆菌病病例，其中大多数不属于同一邮政编码。相反，在偏远地区观察到的病例很少。但是，这些案例大多数都属于同一邮政编码，这表明可能存在当地疫情。

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  • 作者

    Medeiros, Diane.;

  • 作者单位

    University of Ottawa (Canada).;

  • 授予单位 University of Ottawa (Canada).;
  • 学科 Health Sciences Immunology.; Biology Microbiology.; Health Sciences Public Health.
  • 学位 M.Sc.
  • 年度 2002
  • 页码 156 p.
  • 总页数 156
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 预防医学、卫生学;微生物学;预防医学、卫生学;
  • 关键词

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