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首页> 外文学位 >Archaeal ribonuclease P has multiple protein subunits homologous to eukaryotic nuclear ribonuclease P subunits (Methanothermobacter thermoautotrophicus).
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Archaeal ribonuclease P has multiple protein subunits homologous to eukaryotic nuclear ribonuclease P subunits (Methanothermobacter thermoautotrophicus).

机译：古细菌核糖核酸酶P具有与真核核糖核酸酶P亚单位（嗜热甲烷杆菌）相似的多个蛋白质亚单位。

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摘要

RNase P removes the 5′ leader from all transfer RNA. RNase P consists of a catalytic RNA molecule and a variable protein content. The 120 kDa RNA component from Bacteria is catalytic in vitro in the absence of its single 14 kDa protein. RNase P from the eukaryotic nucleus has more protein (nine proteins in yeast) and an RNA that displays no activity without its protein. The Archaea have RNase P RNAs similar in structure to those of Bacteria. However, many of these RNAs display no catalytic activity in vitro without their proteins, and those that are catalytic require extraordinarily high ionic concentrations.; Methanothermobacter thermoautotrophicus RNase P has an RNA strikingly similar in structure to bacterial RNase P RNA, but requires ∼3M monovalent cations and 300 mM Mg2+ for activity in vitro without its protein. The protein subunit of RNase P from Bacillus subtilis can reconstitute with the RNase P RNA from M. thermoautotrophicus at low ionic strengths. However, there is no open reading frame in the M. thermoautotrophicus genome with similarity to a bacterial RNase P subunit, and M. thermoautotrophicus RNase P has a buoyant density in Cs2SO4 of 1.42 g/ml, suggesting more protein than bacterial RNase P. There are four open reading frames (ORFs) in M. thermoautotrophicus, MTH11, MTH687, MTH688 and MTH1618, with similarity to the yeast RNase P subunits Pop4p, Pop5p, Rpp1p and Rpr2p, respectively, and these have homologs in other Archaea. Antibodies against these proteins detect them in western blots of purified M. thermoautotrophicus RNase P, and can immunoprecipitate RNase P activity. Yeast two-hybrid analyses have demonstrated in vivo interactions between MTH688 and MTH687, corresponding to findings with human and yeast nuclear RNase P proteins, and between MTH1618 and MTH11, corresponding to similar findings with yeast nuclear RNase P subunits. These results demonstrate that the archaeal RNase P holoenzyme has protein subunits homologous to eukaryotic nuclear RNase P, and also suggest that the overall architecture of the enzymes are similar.
机译：RNase P从所有转移RNA中去除了5 '前导序列。 RNase P由催化性RNA分子和可变的蛋白质含量组成。细菌的120 kDa RNA成分在没有单个14 kDa蛋白的情况下在体外具有催化作用。真核细胞核糖核酸酶P具有更多的蛋白质（酵母中有九种蛋白质），而没有其蛋白质则无活性的RNA。古细菌具有与细菌相似的RNase P RNA。然而，这些RNA中的许多在没有它们的蛋白质的情况下在体外没有显示出催化活性，并且那些具有催化作用的RNA需要非常高的离子浓度。 嗜热甲烷嗜热自养菌 RNase P具有与细菌RNase P RNA极为相似的RNA结构，但需要约3M单价阳离子和300 mM Mg 2 + 来进行体外活性。 不含蛋白质。来源于枯草芽孢杆菌的RNase P蛋白亚基可以在低离子强度下与来自 M.autoautotrophicus 的RNase P RNA重组。但是， M中没有开放阅读框。嗜热自养菌基因组与细菌RNase P亚基和 M相似。嗜热自养菌 RNase P在Cs 2 SO 4 中的浮力密度为1.42 g / ml，表明蛋白质比细菌RNase P多。有四个开放阅读框（斜体）中的（ORF）。嗜热自养菌，MTH11，MTH687，MTH688和MTH1618，分别与酵母RNase P亚基Pop4p，Pop5p，Rpp1p和Rpr2p相似，并且在其他古细菌中具有同源性。针对这些蛋白质的抗体在纯化的 M的Western印迹中检测到它们。嗜热自养核糖核酸酶P，可以免疫沉淀核糖核酸酶P的活性。酵母双杂交分析表明，MTH688和MTH687之间的体内相互作用对应于人类和酵母核RNase P蛋白的发现，MTH1618和MTH11之间的相互作用对应于酵母核RNase P亚基的相似发现。 。这些结果表明，古细菌RNase P全酶具有与真核RNase P同源的蛋白质亚基，并且还暗示了酶的总体结构是相似的。

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  • 作者

    Hall, Thomas Andrew.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Biology Molecular.; Biology Microbiology.; Chemistry Biochemistry.
  • 学位 Ph.D.
  • 年度 2001
  • 页码 154 p.
  • 总页数 154
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子遗传学;微生物学;生物化学;
  • 关键词

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