• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >The Screening of One-Bead-One-Compound (OBOC) Small Molecule Libraries against Phage Display Libraries -The Development of a Novel Multiplex Screening Approach and its Applications.
【24h】

The Screening of One-Bead-One-Compound (OBOC) Small Molecule Libraries against Phage Display Libraries -The Development of a Novel Multiplex Screening Approach and its Applications.

机译：针对噬菌体展示库的一珠一化合物（OBOC）小分子文库的筛选-一种新型多重筛选方法的开发及其应用。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Binding, the non-covalent interaction between biomolecules or the interaction between ligands and receptors can either enhance or inhibit biological functions. More and more ligands have been discovered and some of them have been developed as therapeutic agents. Besides, several protein tags and their corresponding ligands, such as GST and glutathione, have been utilized for affinity purification, immobilization of tagged proteins, or the labeling of the tagged proteins with fluorophore. While the demand of exploring novel therapeutic targets, therapeutic agents, and protein tagging systems is increasing, instead of screening either OBOC or PHD libraries against a single target, my study is focusing on the development of a approach of screening PHD libraries against OBOC small molecule library. This novel multiplex screening method is employed in two applications in my studies. One is to screen the OBOC small molecule library against phage display human tumor proteome library to identify novel drugable targets and their corresponding therapeutics at the same time. A small molecule inhibitor against Human translation initiation factor 5B, EIF5B, was discovered and can be a valuable tool to dissect the effect of translation regulation. The other one is to screen the same OBOC small molecule library against a phage display random peptide library. A peptide-small molecule binding partner was discovered through the screening and affinity optimization. The peptide can be used to tag the protein of interest and the small molecule can be used to probe the tagged protein. As more peptide-small molecule binding pairs will be discovered through this novel screening strategy, these binding pairs can be utilized to develop multi-color probing system to visualize in vivo protein colocalization in cells.
机译：结合，生物分子之间的非共价相互作用或配体与受体之间的相互作用可以增强或抑制生物学功能。已发现越来越多的配体，其中一些已被开发为治疗剂。此外，几种蛋白质标签及其相应的配体，例如GST和谷胱甘肽，已被用于亲和纯化，固定标记的蛋白质或用荧光团标记标记的蛋白质。在探索新型治疗靶标，治疗剂和蛋白质标签系统的需求不断增长的同时，我不是针对单一靶标筛查OBOC或PHD文库，而是将研究重点放在针对OBOC小分子筛查PHD文库的方法上图书馆。这种新颖的多重筛选方法在我的研究中有两个应用。一种方法是针对噬菌体展示人类肿瘤蛋白质组库筛选OBOC小分子文库，以同时鉴定新型可药物治疗靶标及其相应的治疗药物。发现了一种针对人类翻译起始因子5B的小分子抑制剂EIF5B，它可以作为分析翻译调控作用的有价值的工具。另一种是针对噬菌体展示随机肽库筛选相同的OBOC小分子库。通过筛选和亲和力优化发现了肽-小分子结合伴侣。该肽可用于标记目的蛋白，而小分子可用于探测标记的蛋白。通过这种新颖的筛选策略，将发现更多的肽-小分子结合对，这些结合对可用于开发多色探测系统，以可视化体内蛋白质在细胞中的共定位。

著录项

  • 作者

    Wu, Chun-Yi.;

  • 作者单位

    University of California, Davis.;

  • 授予单位 University of California, Davis.;
  • 学科 Health Sciences Toxicology.;Chemistry Pharmaceutical.;Health Sciences Pharmacology.
  • 学位 Ph.D.
  • 年度 2010
  • 页码 198 p.
  • 总页数 198
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号