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首页> 外文学位 >Analysis of trans-epithelial electrical resistance (TEER) in organ-on-chips to study the functions of human placenta.
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Analysis of trans-epithelial electrical resistance (TEER) in organ-on-chips to study the functions of human placenta.

机译：分析片上器官中的跨上皮电阻（TEER），以研究人类胎盘的功能。

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摘要

Organ-on a-chip technology is becoming a popular method for drug testing. Microfluidic Organ-on-a-chip eliminates the need for live objects, such as animals, to do drug testing. Although the Organ-on-a-chip devices are becoming popular, creating a microfluidic device to represent human placenta is more challenging than other organs. Current drug testing methods are considered unethical and unreliable due to drug testing on animals and cost. The human placenta is a temporary organ created during pregnancy to connect fetus and its mother to allow nutrient supply, gas exchange, waste elimination, and avoid internal infections. Researchers wanted to study how the two-way traffic reacts when it's blocked by bacteria/viruses while it transfers the nutrients/oxygen, since the lack of transportation can affect the health of the mother and the fetus. When animal organs are used for testing on the human placenta, inconsistent results have been found due to the differences among species in physiological functions of human placental barrier, including permeability, transport of supplements, patterns of blood flow, and even in metabolic activities. Our "Placenta-on-a-chip" device is designed to represent a working placenta organ using human cells in order to imitate the nutrient/waste transfer between the maternal blood and fetal blood. HUVEC cells (endothelial) and BeWo cells (trophoblast) were used to represent the placental barrier with a high concentrated collagen coated membrane. The objective is to analyze the glucose transport between the endothelial-trophoblast barrier through the membrane. In the future, researchers will have the opportunity to use the placenta-on-a-chip model for additional research in drug testing.
机译：Organ-on a-chip技术正成为一种流行的药物测试方法。芯片上的微流体器官消除了对诸如动物之类的活体进行药物测试的需要。尽管片上器官装置变得越来越流行，但制造代表人类胎盘的微流体装置比其他器官更具挑战性。由于对动物进行药物测试和成本，当前的药物测试方法被认为是不道德和不可靠的。人胎盘是在怀孕期间创建的一个临时器官，用于连接胎儿及其母亲，以提供营养，气体交换，消除废物并避免内部感染。研究人员想研究双向交通在转移营养/氧气的过程中被细菌/病毒阻止时如何反应，因为缺乏交通会影响母亲和胎儿的健康。当使用动物器官测试人类胎盘时，由于物种之间人类胎盘屏障的生理功能（包括通透性，补充剂的运输，血流模式，甚至是代谢活动）的差异，发现了不一致的结果。我们的“单胎盘胎盘”设备旨在代表使用人体细胞的正常胎盘器官，以模仿母体血液和胎儿血液之间的营养/废物转移。 HUVEC细胞（内皮细胞）和BeWo细胞（滋养细胞）用于代表具有高浓度胶原蛋白包被膜的胎盘屏障。目的是分析内皮-滋养层屏障之间通过膜的葡萄糖转运。将来，研究人员将有机会使用单胎盘胎盘模型进行药物测试的其他研究。

著录项

  • 作者

    Pemathilaka, Rajeendra Lakruwan.;

  • 作者单位

    Iowa State University.;

  • 授予单位 Iowa State University.;
  • 学科 Mechanical engineering.;Biomedical engineering.;Physiology.
  • 学位 M.S.
  • 年度 2016
  • 页码 77 p.
  • 总页数 77
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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