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首页> 外文学位 >The influence of somatotropin and oocyte aspiration on transvaginal ultrasound-guided oocyte recovery and ovarian health in cattle.
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The influence of somatotropin and oocyte aspiration on transvaginal ultrasound-guided oocyte recovery and ovarian health in cattle.

机译：生长激素和卵母细胞吸入对经阴道超声引导的卵母细胞恢复和卵巢健康的影响。

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摘要

The emerging fields of transgenics, cloning, in vitro fertilization, and breed improvement rely on the availability of oocytes of known origin. Somatotropin has been reported to increase ovarian follicular populations in several species. It is less clear whether the number of oocytes which can be aspirated from those follicles is increased correspondingly. The hypothesis that exogenous ST alters the intrafollicular environment in a way which diminishes the ability to non-surgically recover oocytes from yearling beef cattle will be tested. My secondary objective was to assess any potential negative consequences of the oocyte aspiration procedure on the ovarian health or subsequent reproductive efficiency of donor animals, resulting from the impact of repeated ovarian puncture.; Several experiments were conducted using transvaginal ultrasound-guided aspiration technology. Heifers were aspirated from four times each to 19 times each, depending on experiment, using daily or sustained-release ST, along with a decreased nutritional plane, for treatment.; Nutritional plane did not impact follicle number or size distribution, or oocyte number, quality or growth. Likewise, treatment with sustained-release ST (one 500 mg injection every 2 wk) did not alter follicle number or size distribution, or oocyte number or quality. Daily ST injection (33 mug/kg body weight/day) resulted in increased follicle populations without a corresponding increase in oocyte numbers, indicating a suppressive effect of ST on oocyte aspiration. Moreover, the increased follicular populations were observed after one week of ST treatment. Further, follicle sizes and oocyte quality and growth were unchanged by daily ST injection, as was stage of oocyte maturation. In addition, daily ST treatment increased follicular thecal cell layer diameter, while leaving other follicular components unaltered. Aspiration increased short-term ovarian bleeding and scarring at sites of puncture, and delayed the ability of the donor animals to subsequently breed. This research further defines the relationship between folliculogenesis and oocyte maturation, specifically the role that ST may play in the interaction of these two events. Findings may result in adjustment of treatment protocols under consideration in both human and bovine IVF and transgenesis programs.
机译：转基因，克隆，体外受精和品种改良的新兴领域取决于已知来源的卵母细胞的可用性。据报道，促生长素能增加几种物种的卵巢滤泡数量。尚不清楚可以从那些卵泡中吸出的卵母细胞数量是否相应增加。将测试外源性ST改变卵泡内环境从而降低一岁肉牛非手术恢复卵母细胞能力的假说。我的次要目的是评估卵子抽吸手术对卵巢健康或随后的供体动物生殖效率的任何潜在负面影响，这些影响是由于卵巢重复穿刺造成的。使用经阴道超声引导抽吸技术进行了几次实验。根据实验，使用每日或持续释放的ST以及减少的营养面，将小母牛从四次抽到19次。营养平面不影响卵泡数目或大小分布，或卵母细胞数目，质量或生长。同样，用持续释放ST（每2周注射一次500 mg）治疗也不会改变卵泡数目或大小分布，或卵母细胞数目或质量。每日ST注射（33杯/ kg体重/天）导致卵泡数量增加，而卵母细胞数量却没有相应增加，表明ST对卵母细胞抽吸具有抑制作用。而且，ST治疗一周后观察到卵泡数量增加。此外，卵泡大小，卵母细胞质量和生长在每日ST注射下均未改变，卵母细胞成熟阶段也是如此。此外，每日ST治疗增加了卵泡鞘细胞层的直径，而其他卵泡成分则保持不变。抽吸增加了短期卵巢出血和穿刺处的瘢痕形成，并延迟了供体动物随后繁殖的能力。这项研究进一步定义了卵泡形成与卵母细胞成熟之间的关系，特别是ST在这两个事件的相互作用中可能发挥的作用。这些发现可能会导致人和牛IVF和转基因计划中正在考虑的治疗方案发生调整。

著录项

  • 作者

    Tripp, Mark William.;

  • 作者单位

    The University of Connecticut.;

  • 授予单位 The University of Connecticut.;
  • 学科 Biology Animal Physiology.
  • 学位 Ph.D.
  • 年度 2000
  • 页码 158 p.
  • 总页数 158
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生理学;
  • 关键词

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