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首页> 外文学位 >Hyperspectral Fourier transform spectrometer for white light reflection spectroscopy and spectral self interference fluorescence microscopy .
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Hyperspectral Fourier transform spectrometer for white light reflection spectroscopy and spectral self interference fluorescence microscopy .

机译：高光谱傅里叶变换光谱仪用于白光反射光谱和光谱自干涉荧光显微镜。

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摘要

This dissertation describes the design, build, and test of a hyperspectral Fourier transform spectrometer (HS-FTS) intended for biological imaging and sensing studies on surfaces. The HS-FTS has two modes of operation: a white light reflectance spectroscopy (WLRS) mode and a spectral self interference fluorescence microscopy (SSFM) mode. WLRS is used to determine an optical thickness profile of biological material bound to a multilayer surface by exploiting the spectral content of light reflected from the surface. WLRS can be used on its own as a label-free detection technique. SSFM is used to establish the average height of fluorophores bound to a specific site on the biomolecules immobilized on the surface by making use of the spectral content of light emitted by the fluorophores. The two techniques can be used in concert to predict molecular conformation of the biomolecules.;Prior to the initiation of this research, the WLRS/SSFM hybrid approach had been demonstrated as a single point measurement where spatial information was obtained by laterally scanning the sample. The HS-FTS improves upon the previous work by enabling simultaneous spectral measurement over all spatial resolution elements, thereby increasing the speed at which a surface can be profiled and also minimizing lateral errors due to movement of the sample. The HS-FTS offers a number of advantages over other hyperspectral imaging technologies such as pushbroom gratings, tunable filters, and computed tomography systems. These advantages include the spectral/spatial multiplex which yields a radiometric benefit, ease of spectral band selection, the ability to operate over large spectral ranges without order sorting issues, and superior background rejection owing to the synchronous detection scheme.;This dissertation presents WLRS and SSFM measurement concepts along with background on Fourier transform spectroscopy. The design, build, characterization, and demonstration of the instrument are then described in detail. Characterization includes WLRS and SSFM measurements of photolithographically etched silicon dioxide on silicon substrates. Data reduction techniques in spectral and temporal spaces are presented and demonstrated on these samples. Biological demonstrations include static measurements of protein spots on surfaces in both operating modes as well as dynamic measurements of DNA spots on specialized polymer surfaces in SSFM mode.
机译：本文介绍了用于表面生物成像和传感研究的高光谱傅里叶变换光谱仪（HS-FTS）的设计，构建和测试。 HS-FTS具有两种操作模式：白光反射光谱（WLRS）模式和光谱自干扰荧光显微镜（SSFM）模式。 WLRS用于通过利用从表面反射的光的光谱含量来确定绑定到多层表面的生物材料的光学厚度分布。 WLRS可以单独用作无标签检测技术。 SSFM用于通过利用荧光团发出的光的光谱含量来确定与固定在表面上的生物分子上特定位置结合的荧光团的平均高度。可以同时使用这两种技术来预测生​​物分子的分子构象。在开始这项研究之前，已经证明WLRS / SSFM混合方法是单点测量，其中通过横向扫描样品获得空间信息。 HS-FTS通过在所有空间分辨率元素上同时进行光谱测量，从而提高了表面轮廓的速度，并使由于样品移动而引起的横向误差最小，从而改进了先前的工作。 HS-FTS与其他高光谱成像技术（例如推扫光栅，可调滤波器和计算机断层扫描系统）相比具有许多优势。这些优点包括：光谱/空间多路复用，具有放射学上的优势；易于选择光谱带；能够在大光谱范围内操作而不会出现阶次排序问题；以及由于同步检测方案而具有出色的背景抑制性能。 SSFM测量概念以及傅立叶变换光谱的背景知识。然后详细描述了仪器的设计，制造，表征和演示。表征包括在硅基板上进行光刻蚀刻的二氧化硅的WLRS和SSFM测量。在这些样本上展示并演示了频谱和时间空间中的数据缩减技术。生物演示包括在两种操作模式下静态测量蛋白质表面上的蛋白斑点，以及在SSFM模式下动态测量专用聚合物表面上的DNA斑点。

著录项

  • 作者

    Dupuis, Julia Rentz.;

  • 作者单位

    Boston University.;

  • 授予单位 Boston University.;
  • 学科 Chemistry Analytical.;Engineering Electronics and Electrical.
  • 学位 Ph.D.
  • 年度 2009
  • 页码 149 p.
  • 总页数 149
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;无线电电子学、电信技术;
  • 关键词

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