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Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry

机译:基于结构光照明和傅里叶变换干涉仪的深度分辨高光谱成像光谱仪

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摘要

A depth resolved hyperspectral imaging spectrometer can provide depth resolved imaging both in the spatial and the spectral domain. Images acquired through a standard imaging Fourier transform spectrometer do not have the depth-resolution. By post processing the spectral cubes (x, y, λ) obtained through a Sagnac interferometer under uniform illumination and structured illumination, spectrally resolved images with depth resolution can be recovered using structured light illumination algorithms such as the HiLo method. The proposed scheme is validated with in vitro specimens including fluorescent solution and fluorescent beads with known spectra. The system is further demonstrated in quantifying spectra from 3D resolved features in biological specimens. The system has demonstrated depth resolution of 1.8 μm and spectral resolution of 7 nm respectively.
机译:深度分辨高光谱成像光谱仪可以在空间和光谱域中提供深度分辨成像。通过标准成像傅立叶变换光谱仪获取的图像没有深度分辨率。通过在均匀照明和结构化照明下对通过Sagnac干涉仪获得的光谱立方(x,y,λ)进行后处理,可以使用结构化照明算法(例如HiLo方法)恢复具有深度分辨率的光谱分辨图像。拟议的方案已通过体外样本验证,包括荧光溶液和具有已知光谱的荧光珠。该系统在从生物样本中的3D解析特征量化光谱中得到了进一步证明。该系统已证明深度分辨率分别为1.8μm和光谱分辨率为7 nm。

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