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首页> 外文学位 >Genetic characterization and pathobiological studies of bovine group A, B and C rotaviruses.
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Genetic characterization and pathobiological studies of bovine group A, B and C rotaviruses.

机译：牛A，B和C组轮状病毒的遗传特征和病理生物学研究。

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摘要

Group A rotaviruses cause diarrhea in young children and animals, including calves. Both group B and C rotaviruses occur in sporadic cases of diarrhea in young calves and adult cattle. Characterization of the VP7 (G type) and VP4 (P type) genes of group A bovine rotaviruses (BRV) from field samples was performed using RT-PCR/RFLP analysis. When these samples were analyzed for G and P type specificity, the P[5]G6 type was most prevalent accounting for 46.7% (41/86) of the samples; 12.8% (11/86) were P[11]G10; 7% (6/86) were P[5]G10 and an equal number were P[11]G6 subtype. The VP7 genes of the G6 subtypes C-8336 and MC27 showed high homology to each other (∼98%) and with other bovine G6 subtypes and showed lower, but substantial homology (80--87% nucleotide sequence homology) with human G6 subtypes and prototype G6 BRVs. Serologically, the C-8336 strain was neutralized by a G6 monoclonal antibody (IC3) and showed 4- to 16-fold differences in antibody titers with G6 BRV NCDV and IND in 2-way neutralization tests.;Ninety calf diarrhea fecal samples and 81 adult cow diarrhea fecal samples (winter dysentery) were tested for group B rotaviruses. By RT-PCR, five (5.5%) calf samples and 15 (18.5%) adult cow diarrhea samples were group B rotavirus; positive. The VP7 genes of WD634tc, ATI and Mebus group B BRVs showed high (over 90%) nucleotide and deduced amino acid homologies among them, and lower homologies (45--57%) with interspecies group B rotaviruses. The WD653, ATI and Mebus strains caused diarrhea in gnotobiotic calves within 3 days post-inoculation and group B rotaviruses were detected in feces by RT-PCR, PAGE and IEM.;WD534tc group C rotavirus was isolated in monkey kidney (MA104) cells from a diarrheic adult cow fecal sample. Genetically and serologically, the WD534tc/C strain was more closely related to the Cowden porcine group C strain than to the Shintoku bovine strain. When the pathogenesis of WD534tc/C alone or combined with group A BRV was examined in gnotobiotic calves, the coinfection of calves with virulent BRV IND/A enhanced fecal shedding of the bovine group C rotavirus and the extent of histopathological lesions in the intestine. These findings suggest a potential new, novel hypothesis involving dual infection for the adaptation of heterologous rotaviruses to new host species.
机译：A组轮状病毒会导致幼儿和小牛（包括小牛）腹泻。 B和C组轮状病毒均发生在小牛和成年牛的腹泻偶发病例中。使用RT-PCR / RFLP分析对A组牛轮状病毒（BRV）的VP7（G型）和VP4（P型）基因进行表征。当分析这些样品的G和P型特异性时，P [5] G6型最为普遍，占样品的46.7％（41/86）；其中P [5] G6型最常见。 P [11] G10为12.8％（11/86）； P [5] G10为7％（6/86），P [11] G6亚型为同等数量。 G6亚型C-8336和MC27的VP7基因彼此之间（〜98％）以及与其他牛G6亚型的同源性很高，与人G6亚型的同源性较低，但有实质性同源性（80--87％核苷酸序列同源性）。和原型G6 BRV。血清学上，C-8336菌株被G6单克隆抗体（IC3）中和，并在2次中和试验中显示出与G6 BRV NCDV和IND的抗体效价差异为4至16倍。; 90头小牛腹泻的粪便样品和81对成年牛腹泻的粪便样本（痢疾）进行了B组轮状病毒检测。通过RT-PCR，B组轮状病毒为5只（5.5％）小牛样品和15只（18.5％）成年牛腹泻样品。正。 WD634tc，ATI和Mebus B组BRV的VP7基因显示出高（超过90％）的核苷酸和推导的氨基酸同源性，而与种间B组轮状病毒的同源性较低（45--57％）。 WD653，ATI和Mebus菌株在接种后3天内引起了致病性小牛腹泻，并通过RT-PCR，PAGE和IEM在粪便中检测到B组轮状病毒； WD534tc C组轮状病毒分离自猴肾（MA104）细胞腹泻的成年牛粪便样本。在遗传和血清学上，WD534tc / C菌株与Cowden猪C组菌株的关系比与Shintoku牛菌株的关系更紧密。当在致病性小牛中检查WD534tc / C单独或与A组BRV结合的发病机理时，牛与强毒BRV IND / A的共感染增强了牛C型轮状病毒的粪便脱落，并增加了肠道中的组织病理学病变程度。这些发现提出了潜在的新的假说，涉及双重感染以使异源轮状病毒适应新的宿主物种。

著录项

  • 作者

    Chang, Kyeong-Ok.;

  • 作者单位

    The Ohio State University.;

  • 授予单位 The Ohio State University.;
  • 学科 Biology Microbiology.;Biology Veterinary Science.;Agriculture Animal Pathology.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 205 p.
  • 总页数 205
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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