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首页> 外文学位 >Role of brome mosaic virus and cucumber mosaic virus coat proteins in viral movement.
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Role of brome mosaic virus and cucumber mosaic virus coat proteins in viral movement.

机译：溴花叶病毒和黄瓜花叶病毒外壳蛋白在病毒运动中的作用。

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摘要

Brome mosaic bromovirus (BMV) and cucumber mosaic cucumovirus (CMV) are genetically related members of the plant virus family Bromoviridae . While these two viruses are very similar in their genome organization, they exhibit differences in viral-host interactions and their requirements for viral spread. BMV has adapted to infecting mainly monocots and a few dicotyledenous hosts. CMV has a very wide host range and equally infects both monocots and dicots. To evaluate the extent to which BMV coat protein (CP) gene is involved in cell-to-cell movement, Chenopodium quinoa plants were inoculated with BMV wild type (wt) RNAs 1 and 2 and a RNA3 variant containing either the beta-glucoronidase (GUS) reporter gene in place of its CP gene or a large deletion in the CP open reading frame (ORF). GUS expression from the BMV CP variant was at all times restricted to initially infected single cells. Application of fluorescence in situ hybridization (FISH) to leaves inoculated with the BMV CP deletion variant demonstrated that resulting infections were subliminal. FISH proved a suitable technique for differentiating subliminal infection sites from cell-to-cell spread. Observing FISH-treated leaves with confocal laser scanning microscopy (CLSM) showed that subliminal infection sites resulting from inoculations with the BMV CP deletion variant did not expand beyond a single cell type, whereas a wt BMV infection could spread to several. These results show that CP, probably in an encapsidation-competent form, along with movement protein (MP), is required for cell-to-cell spread of BMV. Both, BMV and CMV require their CPs for systemic spread, however the form in which CP is involved in cell-to-cell movement is distinct. The N-terminal basic arm of CMV CP contains an arginine-rich cluster, characteristic of RNA binding proteins, which is conserved among BMV and other plant and non-plant viruses. To identify regions of the CMV CP N-terminus that are essential for viral RNA interactions, a set of CMV CP variants was constructed and the behavior of each variant with respect to infectivity, packaging, and spread was examined. Biological assays in C. quinoa and Nicotiana benthamiana and progeny analysis revealed that a major portion of the CMV CP N-terminus is dispensable for CP-RNA interactions and established that CMV can move cell-to-cell in a non-virion form, while encapsidation is obligatory for long distance spread.
机译：凤梨花叶病毒（BMV）和黄瓜花叶黄瓜花叶病毒（CMV）是植物病毒科Bromoviridae的遗传相关成员。尽管这两种病毒的基因组组织非常相似，但它们在病毒与宿主之间的相互作用以及对病毒传播的要求方面却表现出差异。 BMV已适应主要感染单子叶植物和一些双子叶植物的宿主。 CMV的宿主范围非常广，并且可以同时感染单子叶植物和双子叶植物。为了评估BMV外壳蛋白（CP）基因参与细胞间移动的程度，将藜麦藜（Chenopodium quinoa）植物接种了BMV野生型（wt）RNA 1和2以及含有β-葡萄糖苷酸酶（ （GUS）报告基因代替其CP基因或CP开放阅读框（ORF）中的大量缺失。来自BMV CP变体的GUS表达始终限于最初感染的单个细胞。将荧光原位杂交（FISH）应用于接种了BMV CP缺失变异体的叶片，表明所产生的感染是阈下的。 FISH被证明是区分潜意识下感染部位与细胞间传播的合适技术。用共聚焦激光扫描显微镜（CLSM）观察用FISH处理的叶片，发现接种BMV CP缺失变体导致的潜意识下感染部位并未扩展到单一细胞类型以外，而wt BMV感染可能扩散到几种。这些结果表明，BMV在细胞之间的传播需要CP以及可能带有外壳蛋白的形式以及运动蛋白（MP）。 BMV和CMV都需要它们的CP进行系统扩散，但是CP参与细胞间移动的形式却截然不同。 CMV CP的N端基本臂包含一个富含精氨酸的簇，该簇具有RNA结合蛋白的特征，在BMV与其他植物和非植物病毒之间是保守的。为了鉴定对于病毒RNA相互作用必不可少的CMV CP N端区域，构建了一组CMV CP变体，并检查了每个变体在传染性，包装和传播方面的行为。奎奴亚藜和本氏烟草的生物学分析以及后代分析表明，CMV CP N末端的大部分对于CP-RNA相互作用是必不可少的，并确定了CMV可以非病毒体形式在细胞间移动，而衣壳化对于长距离传播是必须的。

著录项

  • 作者

    Schmitz, Isabelle.;

  • 作者单位

    University of California, Riverside.;

  • 授予单位 University of California, Riverside.;
  • 学科 Biology Botany.;Agriculture Plant Pathology.;Biology Microbiology.
  • 学位 Ph.D.
  • 年度 1999
  • 页码 152 p.
  • 总页数 152
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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