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首页> 外文学位 >I. Steroid hormone enrichment of brine shrimp (Artemia salina) nauplii. II. Factors associated with acute bacterial infection that limit fertility.
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I. Steroid hormone enrichment of brine shrimp (Artemia salina) nauplii. II. Factors associated with acute bacterial infection that limit fertility.

机译：I.盐水虾（卤虫）无节幼体中类固醇激素的富集。二。与急性细菌感染有关的限制生育的因素。

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摘要

This experiment was conducted to determine the effect of incubation of Artemia nauplii with steroids prior to the first feeding instar II stage of Artemia development. Decapsulated Artemia cysts were incubated at a concentration of 3 g cysts/L in hatching media (1 L seawater at 1.030 SG, 0.08 g highly unsaturated fatty acid supplement) and either 20 mg 17beta-estradiol (E2) or 17alpha-methyldihydrotestosterone (MDHT) dissolved in 3 mL 70% ethyl alcohol or ethyl alcohol alone (control). Cysts were cultivated at 18--20°C for 24h. Nauplii were collected by filtering culture media, and were rinsed in 1 L fresh water. Artemia cultivated in control media contained 0.07 +/- 0.01 ng E 2/mg dry weight, and E2-treated Artemia contained 461 +/- 40 ng E2/mg dry weight (p < .0001). MDHT treated Artemia contained 546 +/- 31 pg/mg dry weight and controls were below the level of detection (4pg/mL; p < .0001).;This experiment evaluated the effect of Gram-positive peptidoglycan (PTG) challenge on reproductive performance of ewes. On d 5 post-breeding, ewes were injected I.V. with saline or 15, 30, or 60 mug/kg of sonicated PTG (Streptococcus pyogenes). Each dose of PTG induced fever. Pregnancy rate differed with treatment (p < .01), but was not correlated with incidence of fever. Neither mean nor profile of E 2 differed with treatment (p > .05). Mean and profile of P4 was lower in all treated than in control ewes (p < 0.01). Patterns of LH pulses did not differ; mean LH (ng/mL) was lower in ewes treated with 0, 15, or 30 mug/kg than in ewes treated with 60 mug/kg (p < 0.01). The pattern of cortisol secretion was biphasic and increased in all treated ewes (p < 0.01); mean cortisol was higher in treated than in control (p < 0.01). Maintenance of pregnancy was reduced in ewes with high concentrations of PGFM as compared to ewes with low concentrations of PGFM (p < 0.05).
机译：进行该实验以确定在卤虫发育的第一个进食龄期II阶段之前，将无节幼体与类固醇一起孵育的效果。在孵化培养基（1 L海水1.030 SG，0.08 g高不饱和脂肪酸补充剂）和20 mg 17beta-雌二醇（E2）或17alpha-甲基二氢睾丸激素（MDHT）中，以3 g囊肿/ L的浓度孵育解囊的Artemia囊肿。溶于3 mL 70％乙醇或单独的乙醇中（对照）。囊肿在18--20°C下培养24h。通过过滤培养基收集Nauplii，并用1 L淡水冲洗。在对照培养基中培养的Artemia含有0.07 +/- 0.01 ng E 2 / mg干重，E2处理的Artemia含有461 +/- 40 ng E2 / mg干重（p <.0001）。 MDHT处理的Artemia的干重为546 +/- 31 pg / mg，对照低于检测水平（4pg / mL； p <.0001）。该实验评估了革兰氏阳性肽聚糖（PTG）刺激对生殖的影响母羊的表现。繁殖后第5天，静脉注射母羊。用盐水或15、30或60杯/千克超声PTG（化脓性链球菌）。每剂PTG引起发烧。妊娠率与治疗方法不同（p <.01），但与发烧的发生率无关。 E 2的均值和分布均与治疗无差异（p> .05）。在所有处理中，P4的均值和分布均低于对照母羊（p <0.01）。 LH脉冲的模式没有差异；用0、15或30杯/千克的母羊的平均LH（ng / mL）比用60杯/千克的母羊的LH低（p <0.01）。皮质醇的分泌模式是双相的，并且在所有处理过的母羊中均增加（p <0.01）。治疗组的平均皮质醇水平高于对照组（p <0.01）。与低浓度PGFM的母羊相比，高浓度PGFM的母羊的妊娠维持能力降低（p <0.05）。

著录项

  • 作者

    Barker, Amanda R.;

  • 作者单位

    West Virginia University.;

  • 授予单位 West Virginia University.;
  • 学科 Biology Animal Physiology.;Agriculture Animal Pathology.;Agriculture Fisheries and Aquaculture.
  • 学位 M.S.
  • 年度 1999
  • 页码 78 p.
  • 总页数 78
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

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