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Applications of capillary electrophoresis in pesticide analysis and toxicology studies.

机译：毛细管电泳在农药分析和毒理学研究中的应用。

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摘要

The purpose of this dissertation research was to examine the utility of capillary electrophoresis (CE) for analysis of pesticides and DNA-adducts. The goal was to develop new analytical methods that are inexpensive, compatible with a broad range of analytes and generate little waste.; In this dissertation research, CE was evaluated as a tool to develop new analytical methods that meet the above criteria. The focus was to develop methods for analysis of a number of pesticides, including chiral pesticides and DNA-adducts. A number of methods were developed using micellar electrokinetic chromatography (MEKC), a mode of CE, to separate a variety of pesticides (atrazine, simazine, alachlor, metolachlor, carbendazim metalaxyl, propiconazole, vinclozolin, primisulfuron and triasulfuron. These methods were then successfully used to determine the residues of the above pesticides in water and soil samples at the ppb level. In addition, capillary electrophoresis using laser-induced fluorescence (LIF) detection was evaluated as a means of improving detection limits for analysis of pesticides. A CE/LIF method was developed for analysis of 2,4-D, dicamba and chlorimuron ethyl at the part-per-trillion (ppt) level.; Capillary electrophoresis using cyclodextrins (CDs) as chiral reagents was evaluated to separate pesticide enantiomers and geometric isomers. A number of commercially available cyclodextrins ({dollar}beta{dollar}-CD, {dollar}gamma{dollar}-CD, and their derivatives) were added to the run buffer alone or in combination with a surfactant to obtain resolution of the model pesticide enantiomers/geometric isomers. The model pesticides used in these experiments included imazaquin, diclofop, imazamethabenz, propiconazole, bioallethrin, fenpropathrin, phenothrin, bitertanol, triadimenol, and dimethomorph. The CE methods used to separate the pesticide enantiomers/isomers were simple, rapid, inexpensive and highly efficient.; Finally, CE was evaluated as a new analytical technique for analysis of DNA-adducts (biomarkers of exposure to cancer causing chemicals). A highly sensitive CE method based on laser-induced fluorescence (LIF) detection was developed for analysis of DNA adducts and the CE/LIF method was used to analyze benzo (a) pyrene-dGMP adduct at the femtomole level.
机译：本研究的目的是检验毛细管电泳（CE）在农药和DNA加合物分析中的应用。目标是开发廉价，可与多种分析物兼容且几乎不产生浪费的新分析方法。在本论文研究中，CE被评估为开发满足上述标准的新分析方法的工具。重点是开发分析多种农药的方法，包括手性农药和DNA加合物。使用胶束电动色谱（CEK）开发了多种方法来分离多种农药（阿特拉津，西咪嗪，丙草胺，甲草胺，多菌灵甲霜灵，丙环唑，长效唑啉，伯硫磺隆和三氟磺隆），这些方法均获得成功。用于测定ppb级水和土壤样品中上述农药的残留量，此外，还对使用激光诱导荧光（LIF）检测的毛细管电泳进行了评估，以此作为提高农药分析检出限的一种方法。开发了LIF方法来分析2万亿分之一（ppt）含量的2,4-D，麦草畏和氯嘧磺隆;使用环糊精（CD）作为手性试剂的毛细管电泳被评估为分离农药对映体和几何异构体的方法。将许多市售环糊精（{dollar}β{dollar} -CD，{dollar}γ{dollar} -CD及其衍生物）添加到运行缓冲液中或单独添加到运行缓冲液中。与表面活性剂组合以获得模型农药对映体/几何异构体的拆分。在这些实验中使用的模型农药包括阿马西喹，双氯芬普，伊马西苯咪唑，丙环唑，生物Allethethrin，fenpropathrin，phenothrin，bitertanol，triadimenol和dimethomorph。用于分离农药对映异构体/异构体的CE方法简便，快速，廉价且高效。最后，CE被评估为分析DNA加合物（暴露于致癌化学物质的生物标记）的新分析技术。开发了一种基于激光诱导荧光（LIF）检测的高灵敏度CE方法，用于DNA加合物的分析，CE / LIF方法用于分析fe中苯并（a）--dGMP加合物。

著录项

  • 作者

    Penmetsa, Phanendrakumar Vs.;

  • 作者单位

    North Carolina State University.;

  • 授予单位 North Carolina State University.;
  • 学科 Health Sciences Toxicology.; Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 1997
  • 页码 99 p.
  • 总页数 99
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 毒物学（毒理学）;化学;
  • 关键词

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