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首页> 外文学位 >An improved method for the isolation and characterization of rat intestinal intraepithelial lymphocytes and its application in a model of helminth infection (Trichinella spiralis).
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An improved method for the isolation and characterization of rat intestinal intraepithelial lymphocytes and its application in a model of helminth infection (Trichinella spiralis).

机译：一种改进的大鼠肠道上皮内淋巴细胞分离和鉴定方法及其在蠕虫感染模型中的应用（Trichinella spiralis）。

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摘要

The present study represents the development of an improved method for the isolation and characterization of rat intestinal intraepithelial lymphocytes (IEL) and its application to the examination of cells in Trichinella spiralis infection for which T helper cell IEL function in immunity has been suggested although not proven.; A method of IEL isolation has been developed that allows for the recovery of IEL with sufficient yield and purity to be useful for a variety of studies. The resulting rat IEL population was examined by flow cytometric analysis and was found to include cells of unique phenotypes, including CD8{dollar}alphaalphasp+{dollar} cells, {dollar}ydelta{dollar}TCR{dollar}sp+{dollar} cells, CD4{dollar}sp+{dollar}CD8{dollar}sp+{dollar}cells, CD8{dollar}sp+{dollar}CD45RC{dollar}sp-{dollar} cells, and a previously unreported population of class II MHC{dollar}sp+{dollar} IEL. Three color flow cytometric analysis of IEL identified CD8{dollar}sp+alphabeta{dollar}TCR{dollar}sp+{dollar} (and probably CD45RC{dollar}sp-){dollar} cells as the predominant class II MHC{dollar}sp+{dollar} subset.; Examination of IEL and mesenteric lymph node cells during Trichinella sprialis infection shows that {dollar}alphasb4{dollar} integrin levels are decreased on IEL and L-selectin levels are increased on MLNC on both days one and two post-infection. On day three post-infection an increase in CD4{dollar}sp+{dollar}CD45RC{dollar}sp+{dollar} IEL is detected and persists following expulsion of the parasite. IEL, which appear to be blocked from proliferation under normal circumstances, proliferate on day one post-infection whereas mesenteric lymph node cells do not proliferate. The significance of these alterations in lymphocyte phenotype during T. spiralis infection are unknown but in conjunction with published observations they imply a role for IEL in the immune response to T. spiralis. (Abstract shortened by UMI.)
机译：本研究代表了一种改进的分离和鉴定大鼠肠上皮内淋巴细胞（IEL）的方法的开发，并将其应用于旋毛虫感染的细胞检查中，该方法已被证明尽管尚未证实，但其T辅助细胞具有IEL免疫功能。;已经开发出一种IEL分离方法，该方法允许以足够的产率和纯度回收IEL，可用于各种研究。通过流式细胞术分析对所得的大鼠IEL群体进行检查，发现其包括独特表型的细胞，包括CD8 {dollar} alphaalphasp + {dollar}细胞，ydelta {dollar} TCR {dollar} sp + {dollar}细胞，CD4 {dollar} sp + {dollar} CD8 {dollar} sp + {dollar}细胞，CD8 {dollar} sp + {dollar} CD45RC {dollar} sp- {dollar}细胞以及以前未报告的II类MHC {dollar} sp + {美元} IEL。对IEL进行的三色流式细胞术分析鉴定出CD8 {dollar} sp + alphabeta {dollar} TCR {dollar} sp + {dollar}（以及可能的CD45RC {dollar} sp-）{dollar}细胞是II类MHC {dollar} sp +的主要细胞{dollar}子集。在旋毛虫旋毛虫感染期间检查IEL和肠系膜淋巴结细胞显示，感染后第一天和第二天，MLE上的{dollar} alphasb4 {dollar}整联蛋白水平降低，而MLNC的L-选择蛋白水平升高。感染后第三天，检测到CD4 {dol}} + {dol}} CD45RC {dol}} ++ dol的IEL升高，并在驱除寄生虫后持续存在。正常情况下似乎阻止增殖的IEL在感染后第一天增殖，而肠系膜淋巴结细胞则不增殖。螺旋螺旋体感染期间淋巴细胞表型的这些改变的重要性尚不清楚，但结合已发表的观察结果，它们暗示IEL在螺旋螺旋体的免疫应答中发挥作用。 （摘要由UMI缩短。）

著录项

  • 作者

    Kearsey, Joy Adele.;

  • 作者单位

    Dalhousie University (Canada).;

  • 授予单位 Dalhousie University (Canada).;
  • 学科 Health Sciences Immunology.
  • 学位 M.Sc.
  • 年度 1997
  • 页码 239 p.
  • 总页数 239
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 预防医学、卫生学;
  • 关键词

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