• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文学位 >Part I. Highly sensitive hybridization assays for prostate-specific antigenmRNA based on time-resolved fluorescence and bioluminescence. Part II. Fluorometric and time-resolved immunofluorometric assays for protein-tyrosine phosphatase and kinase activity.
【24h】

Part I. Highly sensitive hybridization assays for prostate-specific antigenmRNA based on time-resolved fluorescence and bioluminescence. Part II. Fluorometric and time-resolved immunofluorometric assays for protein-tyrosine phosphatase and kinase activity.

机译：第一部分：基于时间分辨荧光和生物发光的前列腺特异性抗原mRNA的高灵敏杂交检测。第二部分用于蛋白质酪氨酸磷酸酶和激酶活性的荧光测定法和时间分辨免疫荧光测定法。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bioanalytical assays, namely nucleic acid hybridization assays and immunoassays, provide powerful tools for scientific investigation. In order for such techniques to be useful in a routine clinical setting, the procedures should be highly sensitive, simple and efficient to perform, and adaptable to automation. The objective of my doctoral research was to develop clinically applicable bioanalytical assays for the determination of cancer-associated analytes.;More specifically, the first pan of this DISSERTATION describes hybridization assays which were developed for the detection and quantification of prostate-specific antigen (PSA) mRNA, characteristic of prostate cancer cells. By using the polymerase chain reaction (PCR) combined with time-resolved fluorometric or bioluminescent detection systems, these assays can detect mRNA representative of one PSA-expressing cell amidst one million PSA-negative cells. These methods can facilitate the early detection of prostate cancer cells in the bloodstream, thereby aiding in the correct staging and treatment of prostate cancer patients.;The quantitative PCR method, which was developed by analyzing PSA mRNA in parallel with the mRNA of the housekeeping gene, ;The second part of the DISSERTATION describes fluorometric and time-resolved immunofluorometric assays developed for the determination of the oncogenically relevant protein-tyrosine kinase (PTK) and phosphatase (PTP) activities.;The first assay developed for the determination of PTP activity utilized Tb;For the time-resolved immunofluoromeuic PTP and PTK assays, synthetic substrates containing Tyr residues were immobilized onto microtitre wells. The P-Tyr groups (formed upon incubation with PTK, or remaining following the PTP reaction) were detected using an anti-phosphotyrosine antibody and an alkaline phosphatase-conjugated secondary antibody. The methods proposed here are safer, more practical, and offer superior sensitivity to established isotopic methods.
机译：生物分析测定，即核酸杂交测定和免疫测定，为科学研究提供了强大的工具。为了使此类技术在常规临床环境中有用，该过程应高度敏感，执行起来简单有效，并适合自动化。我的博士研究的目的是开发用于确定癌症相关分析物的临床适用的生物分析检测方法;更具体地说，本论文的第一部分介绍了用于检测和定量前列腺特异性抗原（PSA）的杂交检测方法mRNA，前列腺癌细胞的特征。通过将聚合酶链反应（PCR）与时间分辨荧光或生物发光检测系统结合使用，这些检测方法可以在一百万个PSA阴性细胞中检测代表一个表达PSA的细胞的mRNA。这些方法可以促进血液中前列腺癌细胞的早期检测，从而有助于前列腺癌患者的正确分期和治疗。定量PCR方法，是通过分析PSA mRNA和看家基因的mRNA同时开发的；；《论文》的第二部分描述了用于测定致癌相关蛋白酪氨酸激酶（PTK）和磷酸酶（PTP）活性的荧光测定法和时间分辨免疫荧光测定法。 Tb；对于时间分辨的免疫荧光PTP和PTK分析，将含有Tyr残基的合成底物固定在微量滴定孔上。使用抗磷酸酪氨酸抗体和结合有碱性磷酸酶的二抗检测P-Tyr基团（在与PTK孵育后形成，或在PTP反应后残留）。本文提出的方法更安全，更实用，并且对已建立的同位素方法具有更高的灵敏度。

著录项

  • 作者

    Galvan, Barbara.;

  • 作者单位

    University of Windsor (Canada).;

  • 授予单位 University of Windsor (Canada).;
  • 学科 Biology Molecular.;Health Sciences Immunology.;Chemistry Analytical.
  • 学位 Ph.D.
  • 年度 1996
  • 页码 220 p.
  • 总页数 220
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号